基于生物信息学分析子宫内膜癌组织中LCAT的表达及意义  

Expression of LCAT in endometrial carcinoma and its clinical significance:A study by bioinformatics analysis

作　　者：陈琳 陈丹[1] 马於 许波群[1,2] CHEN Lin;CHEN Dan;MA Yu;XU Boqun(Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210003,China;Department of Obstetrics and Gynecology,Sir Run Run Hospital,Nanjing Medical University,Nanjing 211112,China)

机构地区：[1]南京医科大学第二附属医院妇产科,江苏南京210003 [2]南京医科大学附属逸夫医院妇产科,江苏南京211112

出　　处：《中国肿瘤外科杂志》2024年第5期445-450,共6页Chinese Journal of Surgical Oncology

基　　金：国家自然科学基金(81873820);南京市卫生科技发展专项资金项目(YKK19150)。

摘　　要：目的探究卵磷脂胆固醇脂酰基转移酶(LCAT)基因在子宫内膜癌中的表达情况及其与临床病理特征和预后的相关性。方法通过UALCAN、GEO以及TCGA数据库分析LCAT基因在子宫内膜癌中的表达水平。通过t检验(或Wilcoxon检验)和χ2检验的方法分析子宫内膜癌患者LCAT表达与不同临床病理参数之间的相关性。采用Kaplan-Meier法和Cox回归分析评估LCAT表达和各个临床病理特征与总生存期之间的关系。收集子宫内膜癌及正常子宫内膜组织各15例,实时荧光定量聚合酶链反应技术检测两组组织中LCAT mRNA的表达情况。结果UALCAN、GEO以及TCGA数据库分析,相较于正常子宫内膜组织,子宫内膜癌组织中LCAT表达水平下降(P<0.01),临床样本检测结果与数据库分析结果一致。LCAT低表达与年龄、肿瘤分级、组织学类型及肌层浸润深度相关(P<0.05)。Kaplan-Meier法生存分析表明LCAT基因低表达组总生存期明显短于高表达组(P<0.01),且通过Cox回归分析进一步证明LCAT是子宫内膜癌的独立危险因素,可以预测子宫内膜癌的不良结局(P<0.05)。结论LCAT基因在子宫内膜癌中表达下降,且与不良预后相关,有望作为子宫内膜癌潜在的预后标志物。Objective To investigate the association of the expression of lecithin cholesterol acyltransferase(LCAT)gene with clinicopathological parameters and prognosis in endometrial carcinoma(EC)patients.Methods The expressions of LCAT in EC were analyzed by UALCAN,GEO,and TCGA database.The correlations between LCAT expression and clinicopathologic characteristics were analyzed by the t-test(or Wilcoxon test)and Chi-square test.Kaplan-Meier method and Cox regression analysis were used to evaluate the relationship between clinicopathologic features and overall survival(OS)of EC patients.The expression of LCAT mRNA in 15 endometrial carcinoma tissues and 15 normal endometrial tissues were also assessed by Real-time quantitative polymerase chain reaction(RT-qPCR).Results According to UALCAN,GEO,and TCGA database analysis,the expression of LCAT was significantly decreased in endometrial carcinoma compared to normal endometrium(P<0.01).The results of clinical sample testing were consistent with the results of database analysis.Lower LCAT expression was significantly related to age,tumor grade,histological type,and myometrial invasion(P<0.05).Kaplan-Meier survival analysis revealed a significantly shorter overall survival in the low expression group of LCAT compared to the high expression group(P<0.01).Furthermore,Cox regression demonstrated that LCAT expression could serve as an independent predictor for poor outcome of EC(P<0.05).Conclusions LCAT expression may be associated with the development of EC,and its low expression might be used as a potential prognostic molecular marker of poor survival in EC.

关 键 词：卵磷脂胆固醇脂酰基转移酶 子宫内膜癌 预后 

分 类 号：R737.33[医药卫生—肿瘤]