血清lncRNA PCAT1、miR-128-3p水平与结肠癌患者病理特征及术后肝转移的关系研究  

Study on the relationship between serum LncRNA PCAT1,miR-128-3p levels and pathological characteristics and postoperative liver metastasis in colon cancer patients

作　　者：石彦科 颜廷启[1] 孙伟涛[1] 陈志飞[1] 孙江江[1] 霍浩然[2] 王军委 SHI Yanke;YAN Tingqi;SUN Weitao;CHEN Zhifei;SUN Jiangjiang;HUO Haoran;WANG Junwei(First Department of Emergency Surgery,Handan Central Hospital,Handan 056000,China;Third Department of General Surgery,Handan Central Hospital,Handan 056000,China;Department of Interventional,Handan Central Hospital,Handan 056000,China)

机构地区：[1]邯郸市中心医院急诊外一科,河北邯郸056000 [2]邯郸市中心医院普外三科,河北邯郸056000 [3]邯郸市中心医院介入科,河北邯郸056000

出　　处：《中国肿瘤外科杂志》2024年第5期458-464,共7页Chinese Journal of Surgical Oncology

基　　金：河北省2024年度医学科学研究课题计划(20241159)。

摘　　要：目的探讨血清长链非编码核糖核酸(LncRNA)前列腺癌相关转录因子1(PCAT1)、微小RNA-128-3p(miR-128-3p)水平与结肠癌患者病理特征及术后肝转移的关系。方法选取2019年2月至2021年1月期间邯郸市中心医院收治的接受手术治疗的157例结肠癌患者(结肠癌组)为研究对象,55例同期健康体检者作为对照组。采用实时荧光定量PCR法检测并比较两组血清LncRNA PCAT1、miR-128-3p表达水平,分析结肠癌患者血清LncRNA PCAT1与miR-128-3p表达水平的相关性,分析血清LncRNA PCAT1、miR-128-3p表达水平与结肠癌临床病理特征的关系。随访3年,统计结肠癌术后肝转移发生率,采用单因素和多因素Logistic回归模型分析结肠癌术后肝转移的危险因素,采用受试者工作特征(ROC)曲线分析血清LncRNA PCAT1、miR-128-3p对结肠癌术后肝转移的预测价值。结果与对照组相比,结肠癌组血清LncRNA PCAT1表达水平升高,miR-128-3p表达水平降低(P<0.05);Pearson相关性分析发现结肠癌患者血清LncRNA PCAT1表达水平与血清miR-128-3p呈负相关(r=-0.661,P<0.001)。不同TNM分期患者血清LncRNA PCAT1表达水平比较,Ⅰ期<Ⅱ期<Ⅲ期;血清miR-128-3p表达水平比较,Ⅰ期>Ⅱ期>Ⅲ期(P<0.05)。无淋巴结转移、未侵犯浆膜层相比,有淋巴结转移、侵犯浆膜层的结肠癌患者血清LncRNA PCAT1表达水平均较高,血清miR-128-3p表达水平均较低(P<0.05)。随访3年,结肠癌术后肝转移发生率为25.48%(40/157)。多因素Logistic回归分析显示,TNM分期Ⅲ期、有淋巴结转移、血清LncRNA PCAT1表达水平升高是影响结肠癌术后肝转移的独立危险因素(P<0.05),血清miR-128-3p表达水平升高是保护因素(P<0.05)。ROC曲线分析显示,血清LncRNA PCAT1、miR-128-3p单独及二者联合预测结肠癌术后肝转移的AUC分别为0.761、0.763、0.836,二者联合预测的灵敏度高于单独预测(P<0.05)。结论结肠癌患者血清LncRNA PCAT1高表达、miR-128-3p低表达,两者Objective To investigate the relationship between serum long non-coding RNA(LncRNA)prostate cancer-related transcription factor 1(PCAT1),microRNA-128-3p(miR-128-3p)levels and pathological features and postoperative liver metastasis in colon cancer patients.Methods One hundred and fifty-seven colon cancer patients(colon cancer group)who underwent surgical treatment in Handan Central Hospital from February 2019 to January 2021 were selected as the study subjects,and 55 healthy subjects in the same period were selected as the control group.The expression levels of serum LncRNA PCAT1 and miR-128-3p in two groups were detected and compared by real-time fluorescence quantitative PCR.The correlation between the expression levels of serum LncRNA PCAT1 and miR-128-3p in colon cancer patients was analyzed,and the relationship between the expression levels of serum LncRNA PCAT1 and miR-128-3p and the clinicopathological features of colon cancer was analyzed.After 3 years of follow-up,the incidence of liver metastasis after colon cancer surgery was counted.The risk factors of liver metastasis after colon cancer surgery were analyzed by univariate and multivariate Logistic regression models,and the predictive value of serum LncRNA PCAT1 and miR-128-3p for liver metastasis after colon cancer surgery was analyzed by receiver operating characteristic(ROC)curve.Results Compared with the control group,the expression level of serum LncRNA PCAT1 in colon cancer group increased,while the expression level of miR-128-3p decreased(P<0.05).Pearson correlation analysis showed that serum LncRNA PCAT1 expression level was negatively correlated with serum miR-128-3p in colon cancer patients(r=-0.661,P<0.001).Comparison of serum LncRNA PCAT1 expression levels in patients with different TNM stages showed that StageⅠ<StageⅡ<StageⅢ.Comparison of serum miR-128-3p expression levels showed that StageⅠ>StageⅡ>StageⅢ(P<0.05).Compared with no lymph node metastasis and no serosal invasion,the expression level of serum LncRNA PCAT1 in colon

关 键 词：结肠癌 肝转移 长链非编码RNA前列腺癌相关转录因子1 微小RNA-128-3p 病理特征 

分 类 号：R735.35[医药卫生—肿瘤]