圆红冬孢酵母突变株利用糖蜜生产类胡萝卜素  

Utilization of Molasses for Carotenoids by a Mutant Strain of Rhodosporidium toruloides

作　　者：钟洁 黄子康 李陈曦 祁峰[1] 何文锦[1] ZHONG Jie;HUANG Zikang;LI Chenxi;QI Feng;HE Wenjin(College of Life Sciences,Fujian Normal University,Fuzhou 350117,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117

出　　处：《福建师范大学学报（自然科学版）》2024年第6期78-85,共8页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(32272287);国家糖料产业技术体系项目(CARS-170501);福建省自然科学基金资助项目(2023J01509)。

摘　　要：糖蜜是制糖工业在滤清、浓缩和结晶甘蔗汁后所留下的废料,富含蔗糖、葡萄糖及多种微量元素。圆红冬孢酵母(Rhodosporidium toruloides)是一种能高产油脂和类胡萝卜素的非模式酵母。以甘蔗糖蜜为唯一碳源,通过定向驯化筛选,获得了耐受高浓度糖蜜的R.toruloides驯化株MM10、MM14、MM18。在YNL、TM和MNL培养基中,这3株突变菌株能高效生产圆酵母红素、圆酵母素和β-胡萝卜素等类胡萝卜素。结果表明,用甘蔗糖蜜作为唯一营养源培养R.toruloides,摇瓶培养MM10突变株最终分别收获了质量浓度为3.54 mg·L^(-1)圆酵母红素、2.94 mg·L^(-1)圆酵母素和4.10 mg·L^(-1)β-胡萝卜素,可为甘蔗糖蜜的再利用和基于R.toruloides来源的类胡萝卜素原料生产提供了新思路。Molasses is the waste material left after the sugar industry has filtered,concentrated,and crystallized the pressed sugar cane juice in the sugar production process,which is rich in sucrose and glucose and contains many trace elements.Rhodosporidium toruloides is a non-modal yeast naturally capable of high production of lipids and carotenoids.In this study,we used sugarcane molasses as the sole carbon source to modify R.toruloides by targeted domestication and screening to obtain domesticated R.toruloides strains MM10,MM14,and MM18,which can tolerate high concentrations of molasses.The three domesticated strains were fermented in YNL,TM,and MNL media to produce carotenoids unique to R.toruloides.The experimental data showed that when R.toruloides was cultured with sugarcane waste molasses as the sole nutrient source,the shake flask culture of MM10 strain ultimately harvested 3.54 mg·L^(-1) torularhodin,2.94 mg·L^(-1) torulene,and 4.10 mg·L^(-1)β-carotene,providing a new idea for the reuse of waste molasses and the production of carotenoid raw materials from R.toruloides sources.

关 键 词：圆红冬孢酵母 糖蜜 定向驯化 类胡萝卜素 

分 类 号：Q939.9[生物学—微生物学]