褪黑素改性聚醚醚酮种植体对骨质疏松大鼠骨结合的影响  

Effect of melatonin⁃modified PEEK implant on osteointegration in osteoporotic rats

作　　者：罗桂生 顾腾 李俊俊 王鹏来 张成 袁长永 LUO Guisheng;GU Teng;LI Junjun;WANG Penglai;ZHANG Cheng;YUAN Changyong(School of Stomatology,Xuzhou Medical University,Xuzhou 221004,China)

机构地区：[1]徐州医科大学口腔医学院,江苏徐州221004 [2]徐州医科大学附属口腔医院种植科,江苏徐州221000

出　　处：《口腔医学》2024年第10期734-741,共8页Stomatology

基　　金：徐州市“彭城英才—医学重点人才”培养项目(XWKYHT⁃20230077);江苏省研究生科研与实践创新计划(KYCX22_2973)。

摘　　要：目的探讨聚多巴胺(polydopamine,PDA)涂层辅助的褪黑素(melatonin,MT)改性聚醚醚酮(polyetheretherketone,PEEK)种植体对骨质疏松大鼠骨结合的影响。方法以PDA涂层作为载体将褪黑素黏附到PEEK种植体上,并通过扫描电子显微镜图像、水接触角、傅里叶变换红外光谱及蛋白吸附实验对所得材料进行理化性能分析。将骨质疏松大鼠骨髓间充质干细胞接种在PEEK片的表面并培养,然后对细胞骨架染色并观察细胞黏附形态,使用CCK⁃8实验评估细胞增殖活性,ALP染色分析成骨分化关键酶活性,实时荧光定量PCR检测成骨相关基因COL⁃1、Runx2、OPN、OCN、BMP⁃2、ALP的表达水平。此外,将种植体植入骨质疏松大鼠股骨中,使用Micro⁃CT检测并定量分析种植体表面的骨体积。结果褪黑素成功负载在PEEK上,细胞黏附形态更好,增殖活性和成骨分化能力显著高于对照组(P<0.01)。在大鼠骨质疏松模型中,改性后的PEEK种植体周围有更多的新骨生成(P<0.01)。结论褪黑素改性后的PEEK种植体生物相容性优异,并改善了骨质疏松环境下种植体的骨结合。Objective To explore the effect of melatonin(MT)⁃modified PEEK implant assisted by polydopamine(PDA)coating on osteointegration in osteoporotic rats.Methods MT was adhered to PEEK implants with PDA coating as carrier.The physicochemical properties of the materials were analyzed by SEM image,water contact angle,FTIR and protein adsorption experiment.OVX⁃rBMSCs were inoculated on the surface of PEEK sheet and cultured.The cytoskeleton was stained and cell adhesion morphology was observed.Cell proliferation activity was evaluated by CCK⁃8 assay;key enzyme activities for osteogenic differentiation were analyzed by ALP staining,and expression levels of osteoblast⁃related genes COL⁃1,Runx2,OPN,OCN,BMP⁃2 and ALP were detected by quantitative real⁃time PCR.In addition,implants were implanted into the femur of osteoporotic rats and bone volume on the implant surface was detected and quantified by Micro⁃CT.Results MT was successfully loaded on PEEK;the cell adhesion was better,and the proliferation activity and osteogenic differentiation ability were significantly higher than those of control group(P<0.01).In the rat osteoporosis model,there was more new bone formation around the modified PEEK implant(P<0.01).Conclusion MT⁃modified PEEK implants have excellent biocompatibility and improve osteointegration in an osteoporotic environment.

关 键 词：聚醚醚酮 褪黑素 骨结合 骨质疏松 聚多巴胺 

分 类 号：R782.13[医药卫生—口腔医学]