过敏性鼻炎外周血单个核细胞基因与治疗中药分析  

Analysis of genes of peripheral blood mononuclear cells in allergic rhinitis and treatment with traditional Chinese medicine

作　　者：马伟 丁乐[3] 陈立早[2] 黄玲 韩依伦 卢小叶 MA Wei;DING Le;CHEN Lizao;HUANG Ling;HAN Yilun;LU Xiaoye(Preventive Treatment Department,Yulin Hospital of Chinese Medicine,Yulin 719000;Rehabilitation Medicine Department,Changsha Central Hospital,Changsha 410008;College of Traditional Chinese Medicine,Changsha Medical University,Changsha 410008,China)

机构地区：[1]榆林市中医医院治未病中心,陕西榆林719000 [2]长沙市中心医院康复医学科,湖南长沙410008 [3]长沙医学院中医学院,湖南长沙410008

出　　处：《生物技术》2024年第5期566-575,565,共11页Biotechnology

基　　金：榆林市科技计划项目(YF-2021-71)。

摘　　要：[目的]基于生物信息学方法分析过敏性鼻炎(Allergic Rhinitis,AR)外周血单个核细胞关键基因及通路,并进行治疗中药的关联分析。[方法]分析GSE50223芯片过敏性鼻炎患者外周血单个核细胞差异基因,利用差异基因进行功能、通路与机制的富集分析,筛选出关键靶点并关联相关治疗中药,对干预关键靶点的治疗中药进行性味归经及功效分析。[结果]GSE50223芯片分析获得差异靶点基因684个。差异基因对AR的调控作用主要与TLRs信号通路、PPAR信号通路、IL-17信号通路等相关关键靶点获得10个,分别为CCNA2、TOP2A、CCNB2、CDC20、CDCA8、KIF11、KIF20A、BUB1、CCNB1及KIF2C。关联中药获得153种,其中苦杏仁与红芪关联频数最高。关联中药以寒性与温性为主,以甘味与辛味居多,入足厥阴肝经、手太阴肺经中药居多,以清热药与补虚药占比较大。[结论]基于生信分析发现10个AR中差异表达关键基因,涉及免疫系统、细胞周期、止血等通路机制,筛选出苦杏仁、红芪、艾叶、白果等可能预防和治疗过敏性鼻炎的潜在药物,为AR的分子发病机制和遣方用药提供数据支持。[Objective]To analyze the key genes and pathways in peripheral blood single nucleated cells of allergic rhinitis based on bioinformatics methods,and to carry out the association analysis of therapeutic Chinese medicines.[Method]We analyzed the differential genes in peripheral blood single nucleated cells of patients with allergic rhinitis on the GSE50223 microarray,used the differential genes to enrich the functions,pathways and mechanisms,screened out the key targets and associated with related therapeutic Chinese medicines,and analyzed the properties and efficacy of therapeutic Chinese medicines that intervene in the key targets.[Result]GSE50223 microarray analysis yielded 684 differential target genes.The regulation of AR by differential genes was mainly related to TLRs signaling pathway,PPAR signaling pathway,IL-17 signaling pathway,etc.Ten key targets were obtained,which were CCNA2,TOP2A,CCNB2,CDC20,CDCA8,KIF11,KIF20A,BUB1,CCNB1,and KIF2C,respectively.153 types of traditional Chinese medicines were obtained.The highest frequency of association was between Kuxingren(armeniacae semen amarum)and Hongqi(hedysari radix).The associated Chinese medicines were mainly cold-natured and warm-natured,mostly sweet and pungent,mostly entered the liver meridian of foot queyin and lung meridian of hand taiyin,and were mostly heat-clearing and deficiency-tonifying medicines.[Conclusion]Based on the biosignature analysis,10 differentially expressed key genes in AR were identified,which were involved in the pathway mechanisms of the immune system,cell cycle,and hemostasis,and potential drugs such as Kuxingren(armeniacae semen amarum),Hongqi(hedysari radix),Aiye(artemisiae argyi folium),and Baiguo(ginkgo semen)were screened for possible prevention and treatment of allergic rhinitis,which provided data support for the molecular pathogenesis of AR and the use of traditional Chinese medicines.

关 键 词：生物信息学 过敏性鼻炎 外周血单个核细胞 GSE50223芯片 关键基因 信号通路 分子机制 中药 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]