Runx2、RANKL及GPR48对激素型股骨头坏死发生的预测价值  

Predictive value of Runx2,RANKL and GPR48 in the onset of hormone-induced osteonecrosis of the femoral head

作　　者：王颖[1] 谭春艳 熊武芳 WANG Ying;TAN Chunyan;XIONG Wufang(Department of Laboratory,Suining Central Hospital,Suining 629000,China)

机构地区：[1]遂宁市中心医院检验科,四川遂宁629000

出　　处：《生物技术》2024年第5期608-612,共5页Biotechnology

摘　　要：[目的]探讨Runx2、RANKL及GPR48在大鼠激素型股骨头坏死组织中的表达及对发病的预测价值。[方法]将20只大鼠随机分为模型组和对照组,模型组采用肌肉注射糖皮质激素构建激素型股骨头坏死大鼠模型;建模成功后对两组行HE染色检查,采用蛋白印迹和RT-PCR法检测病灶组织中Runx2、RANKL及GPR48水平;采用logistics多元回归模型分析Runx2、RANKL及GPR48与大鼠激素型股骨头坏死组织发病的关系。[结果]模型组中可见组织细胞萎缩、细胞结构紊乱并可见细胞聚集固缩,且存明显的空骨陷窝;空骨陷窝率、股骨头组织里面的脂肪细胞最大直径方面,模型组的大鼠较对照组大鼠明显高(46.38μm±7.49μm vs 23.81μm±6.18μm;20.93%±2.94%vs 7.67%±1.04%),而骨小梁面积方面则较对照组大鼠低(28.74%±4.53%vs 60.95%±5.07%),且差异明显(P<0.05);模型组大鼠组织中RANKL蛋白和mRNA水平明显高于对照组(5.92%±0.14%vs 1.29%±0.07%;2.89%±0.12%vs 0.21%±0.05%),Runx2、GPR48蛋白水平明显低于对照组(0.91%±0.11%vs 2.83%±0.21%;1.34%±0.12%vs 2.83%±0.09%),且差异有统计学意义(P<0.05);激素型股骨头坏死组织中高RANKL、低Runx2及低GPR48水平是应对大鼠激素型股骨头坏死组织发病的独立性威胁因素,且差异有统计学意义(P<0.05)。[结论]大鼠激素型股骨头坏死组织中Runx2和GPR48处于低表达,RANKL处于高表达状态,且高RANKL、低Runx2及低GPR48水平是应对大鼠激素型股骨头坏死组织发病的独立性威胁因素。[Objective]To analyze the expression of Runx2,RANKL and GPR48 in steroid induced osteonecrosis of the femoral head in rats and their predictive value.[Method]20 rats were randomly divided into model group and control.In model group,glucocorticoid was injected intramuscularly to establish steroid induced femoral head necrosis model.After successful modeling,HE staining was performed in the two groups,and the levels of Runx2,RANKL and GPR48 in the lesion tissue were detected by Western Blotting and RT-PCR.The relationship between Runx2,RANKL and GPR48 and steroid induced femoral head necrosis was analyzed by logistic multiple regression model.[Result]In the model group,tissue cell atrophy,cell structure disorder,cell aggregation and pyknosis were observed,and there were obvious empty bone lacunae;the maximum diameter of adipocytes and empty bone lacunae rate in the model group were obvious higher than control group(46.38μm±7.49μm vs 23.81μm±6.18μm;20.93%±2.94%vs 7.67%±1.04%).And the trabecular area was lower than that control group(28.74%±4.53%vs 60.95%±5.07%),and the difference was statistically obvious(P<0.05);the RANKL protein and mRNA levels in the model group were obvious higher than control group(5.92%±0.14%vs 1.29%±0.07%;2.89%±0.12%vs 0.21%±0.05%).The levels of Runx2,GPR48 were obvious lower than control group(0.91%±0.11%vs 2.83%±0.21%;1.34%±0.12%vs 2.83%±0.09%).The levels of Runx2,RANKL and GPR48 in steroid induced osteonecrosis of the femoral head were independent risk factors for the pathogenesis of steroid induced osteonecrosis of the femoral head(P<0.05).[Conclusion]Runx2 and GPR48 were highly expressed and RANKL was low expressed in rats with steroid induced osteonecrosis of the femoral head.Low Runx2,high RANKL and low GPR48 levels were independent risk factors for the pathogenesis of steroid induced osteonecrosis of the femoral head in rats.

关 键 词：RUNX2 RANKL GPR48 大鼠 激素型股骨头坏死 预测 空骨陷窝 糖皮质激素 

分 类 号：R681.8[医药卫生—骨科学]