MiRNA-200a and miRNA-200b expression,and vitamin-D level:Prognostic significance in obese non-diabetic and obese type 2 diabetes mellitus individuals  

作　　者：Abrar Fahad Alshahrani Fauzia Ashfaq Abdulrahman A Alsayegh Mohammed Bajahzer Mohammad Idreesh Khan Mirza Masroor Ali Beg 

机构地区：[1]Department of Clinical Nutrition,College of Nursing and Health Sciences,Jazan University,Jazan 82817,Saudi Arabia [2]Department of Basic Health Sciences,College of Applied Medical Sciences,Qassim University,Buraydah,51452,P.O.Box 6666 Saudi Arabia [3]Faculty of Medicine,Alatoo International University,Bishkek 720048,Kyrgyzstan

出　　处：《World Journal of Clinical Cases》2024年第36期6916-6925,共10页世界临床病例杂志（英文）

基　　金：Supported by The Deputyship for Research&Innovation,Ministry of Education in Saudi Arabia,for funding this research work through the project number ISP-24.,Jazan University,Jazan 82817,Saudi Arabia.

摘　　要：BACKGROUND Obesity and type 2 diabetes mellitus(T2DM)are frequent co-occurring disorders that affect regular metabolic functions.Obesity has also been linked to an inc-reased risk of developing diabetes.Obesity and diabetes are on the rise,increa-sing healthcare costs and raising mortality rates.Research has revealed that the expression profile of microRNAs(miRNAs)changes as diabetes progresses.Fur-thermore,vitamin D may have an anti-obesity effect and inverse association with body weight and body mass index(BMI).Low vitamin D levels do not solely cause obesity,which could be a factor in the etiology of T2DM.METHODS This study included 210 participants,of which,82 were obese(BMI>30 kg/m2)without T2DM,28 were obese with T2DM,and 100 were healthy controls.BMI was evaluated and both fasting and postprandial blood glucose were used to confirm T2DM.Exosomal miRNA-200a and miRNA-200b expression were analyzed using real-time PCR using Taqman probes,and vitamin-D levels were evaluated using an electrochemiluminescence-based immunoassay technique.All data analyses were performed using SPSS 20.0 and GraphPad Prism 5 software.RESULTS Overall,a 2.20-and 4.40-fold increase in miRNA-200a and miRNA-200b expression was observed among participants compared to healthy controls.MiRNA-200a and miRNA-200b expression among obese participants increased 2.40-fold and 3.93-fold,respectively,while in obese T2DM participants these values were 2.67-fold,and 5.78-fold,respectively,and these differences were found to be statistically significant(P=0.02)(P<0.0001).Obese participants showed a vitamin D level of 34.27 ng/mL,while in obese-T2DM participants vitamin D level was 22.21 ng/mL(P<0.0001).Vitamin D was negatively correlated with miRNA-200a(r=-0.22,P=0.01)and miRNA-200b(r=-0.19,P=0.04).MiRNA-200a sensitivity was 75%,and specificity was 57%,with a cutoff value of 2.07-fold.MiRNA-200b sensitivity was 75%,and specificity was 71%with a cutoff value of 4.12-fold,suggesting that miRNA-200a and miRNA-200b with an increased expression of 2.07-an

关 键 词：OBESITY Type 2 diabetes mellitus MiRNA200a MiRNA200b VITAMIN-D PROGNOSIS 

分 类 号：R587.1[医药卫生—内分泌]