Strategic insights into the cultivation of pancreatic cancer organoids from endoscopic ultrasonography-guided biopsy tissue  

作　　者：Jia-Li Yang Jun-Feng Zhang Jian-You Gu Mei Gao Ming-You Zheng Shi-Xiang Guo Tao Zhang 

机构地区：[1]Institute of Hepatopancreatobiliary Surgery,Chongqing General Hospital,Chongqing University,Chongqing 401147,China

出　　处：《World Journal of Gastroenterology》2024年第42期4532-4543,共12页世界胃肠病学杂志（英文）

基　　金：the Chongqing Talent Plan“Contract System”Project,No.cstc2022ycjh-bgzxm0137;Natural Science Foundation of Chongqing,No.CSTB2024NSCQ-MSX0003;the Ethics Committee of Chongqing General Hospital.The ethics review number:No.KY S2022-045-01.

摘　　要：BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine for advanced PC.AIM To explore how to obtain appropriate puncture tissues from EUS-FNB and optimize the strategy for efficiently constructing PCOs,providing an efficient tool for the advancement of personalized medicine.METHODS Patients who underwent EUS-FNB for the diagnosis of PC tissue were prospectively enrolled.We refined the endoscopic biopsy procedures and organoid cultivation techniques.All tissue specimens verified by on-site pathological assessment were cultured in a semi-suspended medium in a microfluidic environment.We assessed differences in PCOs cultured beyond and below five generations examining patient demographics,specimen and organoid attributes,and the sensitivity of organoids to a panel of clinical drugs through cell viability assays.RESULTS In this study,16 patients with PC were recruited,one sample was excluded because onsite cytopathology showed no tumor cells.Successful organoid generation occurred in 93.3%(14 of 15)of the EUS-FNB specimens,with 60%(9 of 15)sustaining over five generations.Among these patients,those with a history of diabetes,familial cancer,or larger tumors exhibited enhanced PCO expandability.The key factors influencing longterm PCOs expansion included initial needle sample quality(P=0.005),rapid initiation of organoid culture postisolation(P≤0.001),and high organoid activity(P=0.031).Drug sensitivity analysis revealed a partial response in two patients following therapeutic intervention and surgery and stable disease in four patients,indicating a moderate correlation between organoid response and clinical outcomes.CONCLUSION Optimal initial needle sampling,rapid and precise biopsy sample processing,process isolated samples as soon as possible,and sufficient cellular material are crucial for successful cultivating PCOs.High organoid activity is an import

关 键 词：Endoscopic ultrasound-guided fine-needle biopsy Pancreatic cancer organoid Puncture technique Cultivation optimization strategy Drug screening 

分 类 号：R735.9[医药卫生—肿瘤]