花生种皮类黄酮物质的代谢组与转录组分析  

Metabolome and transcriptome analysis of flavonoids in peanut testa

作　　者：金欣欣[1] 苏俏 宋亚辉[1] 杨永庆[1] 李玉荣[1] 王瑾[1] JIN Xin-Xin;SU Qiao;SONG Ya-Hui;YANG Yong-Qing;LI Yu-Rong;WANG Jin(Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences/Hebei Key Laboratory of Crop Genetics and Breeding,Shijiazhuang 050035,Hebei,China)

机构地区：[1]河北省农林科学院粮油作物研究所/河北省作物遗传育种重点实验室,河北石家庄050035

出　　处：《作物学报》2024年第12期2950-2961,共12页Acta Agronomica Sinica

基　　金：财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-13);河北省现代农业产业技术体系建设专项(HBCT2024040101,HBCT2024040204);河北省花生现代种业科技创新团队(21326316D);河北省农林科学院创新工程(2022KJCXZX-LYS-11)资助。

摘　　要：为探究不同颜色花生种皮的类黄酮物质成分及花青素生物合成对种皮颜色形成的调控机制,本研究利用粉色、红色、白色、黑色以及花斑色(红色和白色)共5种颜色差异明显的花生品种,对种皮进行类黄酮代谢组和转录组分析,明确种皮花青素合成相关的关键代谢物和关键基因。结果表明,5种花生种皮共鉴定到329种类黄酮代谢物,其中黄酮醇类物质相对含量及种类最多。共检测到19种花青素色苷,主要是矢车菊素色苷、飞燕草素色苷、矮牵牛素色苷,多以葡萄糖苷、桑布苷、芸香苷、半乳糖苷等糖苷修饰。黑色种皮花青素含量约是其他皮色的22.60~66.72倍。黑色种皮中以矢车菊素-3-O-桑布双糖苷相对含量最高。4种有色种皮分别与白色种皮相比,差异代谢物主要在花青素生物合成、类黄酮生物合成、黄酮和黄酮醇生物合成、异黄酮生物合成途径中显著富集。有色种皮中,类黄酮和花青素生物合成途径结构基因的高表达水平,是促进种皮花青素积累的主要原因。花青素还原酶(ANR)和糖基转移酶(UGT)是参与种皮色素沉着的候选基因,两者的活性以及对底物花青素的竞争,最终决定花生种皮的颜色模式。本研究解析了类黄酮物质对花生种皮颜色合成的调控机制,可为特色花生品种培育以及营养价值利用提供重要参考。To explore the regulatory mechanisms of flavonoid components and anthocyanin biosynthesis in the color formation of peanut testa,we conducted a study using five peanut cultivars with different testa colors:pink,red,white,black,and speckled(red and white).The key metabolites and genes related to anthocyanin biosynthesis were identified using flavonoid metabolomics and transcriptomics.Our results revealed the identification of 329 flavonoid metabolites in peanut testa,with flavonols being the most abundant in both relative content and variety.We detected 19 types of anthocyanidins,including cyanidin,delphinidin,and petunidin.Most anthocyanidins were modified with glucoside,morbuside,rutin,galactoside,and other glycosides.Notably,the anthocyanin content in black testa was 22.60–66.72 times higher than that in other testa colors,with cyanidin-3-O-sambutin being the most prevalent in black testa.Different metabolites were significantly enriched in anthocyanin biosynthesis,flavonoid biosynthesis,flavone and flavonol biosynthesis,and isoflavone biosynthesis pathways in colored testa compared to white testa.The high expression levels of structural genes in the flavonoid and anthocyanin biosynthesis pathways promoted anthocyanin accumulation in colored testa.Anthocyanin reductase(ANR)and glycosyltransferase(UGT)emerged as candidate genes involved in testa pigmentation,with the competition and activity of UGT and ANR against substrate anthocyanin determining the color pattern of peanut testa.These findings elucidate the regulatory mechanisms of flavonoid substances in peanut testa color,providing valuable references for the breeding of special peanut varieties and the utilization of their nutritional value based on color differences.

关 键 词：花生 种皮 类黄酮 代谢组 转录组 

分 类 号：S565.2[农业科学—作物学]