普通菜豆PvIQD基因家族鉴定及其非生物胁迫下的表达分析  

Identification of the PvIQD Gene Family and Its Expression Analysis Under Abiotic Stress in Common Bean(Phaseolus vulgaris)

作　　者：董雪 赵敏 李佳 邱傅懿 王燕 赵建栋 畅建武 郝晓鹏 DONG Xue;ZHAO Min;LI Jia;QIU Fu-Yi;WANG Yan;ZHAO Jian-Dong;CHANG Jian-Wu;HAO Xiao-Peng(Center for Agricultural Genetic Resources Research/Ministry of Agriculture/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops,Shanxi Agricultural University,Taiyuan 030031,China;College of Agriculture,Shanxi Agricultural University,Taiyuan 030031,China)

机构地区：[1]山西农业大学农业基因资源研究中心/杂粮种质资源发掘与遗传改良山西省重点实验室,太原030031 [2]山西农业大学农学院,太原030031

出　　处：《农业生物技术学报》2024年第11期2511-2525,共15页Journal of Agricultural Biotechnology

基　　金：国家现代农业产业技术体系-食用豆(CARS-08);国家重点研发计划(2021YFD1600601);山西省后稷实验室自主立项课题(202304010930003);山西农业大学生物育种工程(YZGC148)。

摘　　要：IQ67结构域(IQ67 domain,IQD)基因编码一类含有IQ67保守结构域的钙调蛋白结合蛋白,在调控植物器官形态和响应逆境胁迫中发挥重要作用。本研究通过生物信息学分析在普通菜豆(Phaseolus vulgaris)基因组中鉴定出34个PvIQD基因,对其理化性质、基因复制、系统进化、基因结构和表达模式等进行相关分析。结果表明,PvIQD基因不均匀地分布在11条染色体上,存在2个片段重复基因对,没有串联重复基因对。Ka/Ks比值均小于1,表明PvIQD基因在进化中受到纯化选择。系统进化分析显示,PvIQD基因家族成员被分为4个亚家族(Ⅰ~Ⅳ),28个PvIQD基因与大豆(Glycine max)的GmIQD基因有一对二的聚类关系。基因结构分析显示,PvIQD基因包含2~6个外显子,大部分PvIQD蛋白序列含有motif1和motif7,同一亚家族具有相似的基因结构和保守基序,IQ基序中的Gln7、Arg11、Gly12、Leu14和Arg16是高度保守氨基酸。共线性分析发现,普通菜豆与双子叶植物拟南芥(Arabidopsis thaliana)和大豆比与单子叶植物水稻(Oryza sativa)和玉米(Zea mays)有更加密切的亲缘关系,与大豆的亲缘关系最为密切。RNA-seq数据分析显示,6个PvIQD基因在不同发育阶段的多个组织中均有高表达,组织特异表达基因PvIQD2和PvIQD19分别在绿色成熟豆荚和根瘤中表达量最高。qRT-PCR结果表明,8个PvIQD基因能够响应干旱和盐胁迫,表现出不同的表达调控模式。本研究为探索PvIQD基因抵御非生物胁迫的调控作用机制提供了参考。The IQ67 domain(IQD)genes encode a class of calmodulin-binding proteins that contain the conserved IQ67 domain.These proteins play important roles in regulating plant organ morphology and responding to adversity stress.In this study,34 PvIQD genes were identified in the genome of common bean(Phaseolus vulgaris)through bioinformatics analysis.Their physicochemical properties,gene duplication events,phylogenetic evolution,gene structures,and expression patterns were analyzed.The study showed,PvIQD genes were unevenly distributed on 11 chromosomes.There were 2 segmentally duplicated gene pairs and no tandem duplicate gene pairs.The Ka/Ks ratios were all less than 1,indicating that PvIQD gene pairs have been under purification selection pressure during evlution.In the phylogenetic analysis,the PvIQD gene family members were divided into 4 subfamilies(Ⅰ~Ⅳ),with 28 PvIQD genes exhibiting a one-to-two clustering relationship with IQD genes from soybean(Glycine max).Gene structure analysis revealed that PvIQD genes contained 2~6 exons,and most PvIQD protein sequences contained motif1 and motif7.PvIQD genes in the same subfamily had similar gene structures and conserved motifs.Gln7,Arg11,Gly12,Leu14,and Arg16 in the IQ motif were highly conserved amino acids.Collinearity analysis showed that common bean had a closer genetic relationship between common bean and dicotyledonous plants(Arabidopsis thaliana and Glycine max),compared with monocotyledonous plants(Oryza sativa and Zea mays),and the closest relationship.Analysis of RNA-seq data showed that 6 PvIQD genes were highly expressed in multiple tissues at different developmental stages.The tissue-specific genes,PvIQD2 and PvIQD19,showed the highest transcript levels in green mature pods and nodules,respectively.The results of qRT-PCR analysis showed that 8 PvIQD genes exhibited distinct expression patterns in response to drought and salt stress.This study provides a reference for exploring the regulatory mechanisms of PvIQD genes in resistance to abiotic stress in com

关 键 词：普通菜豆 IQ67结构域(IQD) 系统进化分析 共线性分析 非生物胁迫 

分 类 号：S529[农业科学—作物学]