Circulating CCRR serves as potential novel biomarker for predicting acute myocardial infarction  

作　　者：Lina Xuan Huishan Luo Shu Wang Guangze Wang Xingmei Yang Jun Chen Jianjun Guo Xiaomeng Duan Xiufang Li Hua Yang Shengjie Wang Hailong Zhang Qingqing Zhang Shulei Liu Yongtao She Kai Kang Lihua Sun 

机构地区：[1]Department of Pharmacology,Harbin Medical University(the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China,Key Laboratory of Cardiovascular Research,Ministry of Education,Joint International Research Laboratory of Cardiovascular Medicine Research,Ministry of Education),College of Pharmacy,State Key Laboratory of Frigid Zone Cardiovascular Diseases(SKLFZCD),Harbin Medical University,Harbin 150000,China [2]Department of Cardiology,the First Affiliated Hospital,Harbin Medical University,Harbin 150000,China [3]Department of Cardiovascular Surgery,the First Affiliated Hospital of Harbin Medical University,Harbin 150000,China [4]NHC Key Laboratory of Cell Transplantation,the First Affiliated Hospital of Harbin Medical University,Harbin 150000,China

出　　处：《Frigid Zone Medicine》2024年第3期137-151,共15页寒地医学（英文）

基　　金：supported by grants from the Natural Science Foundation of China(81970202,81903609);by Natural Science Foundation of Heilongjiang Province,China(LH2022H002);by the Outstanding Young Talent Research Fund of College of Pharmacy,Harbin Medical University(2019-JQ-02);2021(the second batch)Research Funds for affiliated research institutes in Heilongjiang Province(CZKYF2021-2-C013).

摘　　要：Objective:Cold regions exhibit a high prevalence of cardiovascular disease,particularly acute myocardial infarction(AMI),which is one of the leading causes of death associated with cardiovascular conditions.Cardiovascular disease is closely linked to the abnormal expression of long non-coding RNA(lncRNA).This study investigates whether circulating levels of lncRNA cardiac conduction regulatory RNA(CCRR)could serve as a biomarker for AMI.Materials and methods:We measured circulating CCRR from whole blood samples collected from 68 AMI patients and 69 non-AMI subjects.An AMI model was established using C57BL/6 mice.Quantitative reverse transcription PCR(qRT-PCR)was used to assess CCRR expression.Exosomes were isolated from cardiomyocytes,and their characteristics were evaluated using electron microscope and nanoparticle tracking analysis.The exosome inhibitor GW4869 was employed to examine the effect of exosomal CCRR on cardiac function using echocardiography.Protein expression was detected using Western blot and immunofluorescence staining.Results:The circulating level of CCRR was significantly higher in AMI patients(1.93±0.13)than in non-AMI subjects(1.00±0.05,P<0.001).The area under the ROC curve(AUC)of circulating CCRR was 0.821.Similar changes in circulating CCRR levels were consistently observed in an AMI mouse model.Exosomal CCRR derived from hypoxia-induced cardiomyocytes and cardiac tissue after AMI were increased,a change that was reversed by GW4869.Additionally,CCRR-overexpressing exosomes improved cardiac function in AMI.Conclusion:Circulating lncRNA CCRR is a potential predictor of AMI.Exosomal CCRR plays a role in the communication between the heart and other organs through circulation.

关 键 词：acute myocardial infarction lncRNA cardiac conduction regulatory RNA EXOSOME 

分 类 号：R542.22[医药卫生—心血管疾病]