基于miR-210表达探讨电针对脑梗死大鼠氧化应激反应的影响  

Effect of electroacupuncture on oxidative stress in rats with cerebral stroke based on miR-210 expression

作　　者：金彪 兰崴[3] 陈和木[1] JIN Biao;LAN Wei;CHEN Hemu(The First Affiliated Hospital of Anhui Medical University,Hefei 230012,Anhui,China;Hefei Hospital Affiliated to Anhui Medical University(Hefei Second People’s Hospital),Hefei 230012,Anhui,China;College of Acupuncture-Moxibustion and Tuina,Anhui University of Chinese Medicine,Hefei 230012,Anhui,China)

机构地区：[1]安徽医科大学第一附属医院,安徽合肥230012 [2]安徽医科大学附属合肥医院(合肥市第二人民医院),安徽合肥230012 [3]安徽中医药大学针灸推拿学院,安徽合肥230012

出　　处：《现代中西医结合杂志》2024年第17期2353-2358,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：安徽省教育厅自然科学基金重点项目(KJ2021A0573)。

摘　　要：目的探讨电针治疗对脑梗死大鼠氧化应激的影响及其可能机制。方法将70只SD大鼠随机分为假手术组、模型1次组、模型3次组、模型7次组、电针1次组、电针3次组、电针7次组,每组10只。除假手术组外,其余组大鼠均采用改良的Longa线栓法构建可逆性脑梗死模型。模型构建后,电针1次组、电针3次组、电针7次组大鼠分别进行1次、3次、7次电针治疗,对应模型组大鼠在背部非穴位进行电针刺激,均1次/d。各组大鼠末次治疗次日进行神经功能评分;TTC染色观察脑梗死情况,计算脑梗死面积;RT-qPCR检测血清与缺血半暗带区miR-210表达情况,Western blot法检测缺血半暗带区缺氧诱导因子-1α(HIF-1α)蛋白表达情况,试剂盒检测血清与缺血半暗带区超氧化物歧化酶(SOD)活力与丙二醛(MDA)含量。结果模型各组大鼠出现明显梗死区域,神经功能评分降低;电针各组大鼠神经功能评分和脑梗死面积均明显低于同时间点模型各组(P均<0.05),且随着电针治疗次数增加,神经功能和脑梗死面积改善率不断提高。与假手术组比较,模型各组血清与缺血半暗带区miR-210 mRNA相对表达量、SOD活力均明显降低(P均<0.05),缺血半暗带区HIF-1α蛋白相对表达量和血清与缺血半暗带区MDA含量均明显升高(P均<0.05);与同时间点模型各组比较,电针各组血清与缺血半暗带区miR-210 mRNA相对表达量、SOD活力均明显升高(P均<0.05),缺血半暗带区HIF-1α蛋白相对表达量和血清与缺血半暗带区MDA含量均明显降低(P均<0.05)。结论电针治疗能够明显改善脑梗死诱发的神经功能损伤,其机制可能与上调miR-210表达,靶向抑制HIF-1α蛋白表达,从而抑制氧化应激反应有关。Objective It is to investigate the effect of electroacupuncture(EA)on oxidative stress in rats with cerebral stroke and its possible mechanism.Methods Modified Longa method was used to establish a reversible cerebral infarction model.Seventy SD rats were randomly divided into sham operation group,model once group,model 3-time group,model 7-time group,EA-once group,EA 3-time group,EA7-time group,with 10 rats in each group.The rats of all groups except for the sham operation group were used to establish models of reversible cerebral infarction by modified Longa wire ligation method.After the models were established,the rats in the EA-once group,EA 3-time group and EA7-time group were treated with EA for 1,3,and 7 times,respectively,the rats in the model groups were stimulated with EA at non-acupoints on the back for corresponding times,all once daily.On the next day after the last treatment,the scores of neurological function of the rats in each group were assessed;the cerebral infarction was observed by TTC staining,and the cerebral infarction area was calculated;the expression of miR-210 in serum and ischemic ischemic penumbra area was detected by RT-qPCR,the expression of hypoxia-inducible factor-1α(HIF-1α)in ischemic ischemic penumbra area was detected by Western blot,and the activity of superoxide dismutase(SOD)and content of malondialdehyde(MDA)in serum and ischemic ischemic penumbra area were measured by the kit.Results There were obvious infarcted areas with reduced neurological function scores in the rats of the model groups;the neurological function scores and cerebral infarcted areas of the rats in the EA groups were all significantly lower than those in the model groups at the same time point(all P<0.05),and the improvement rates of neurological function and cerebral infarcted areas were continuously improved with the increase of the times of treatments of EA.Compared with the sham operation group,the relative expression of miR-210 mRNA and SOD activity in serum and ischemic penumbra area were signifi

关 键 词：脑梗死 电针疗法 氧化应激 MIR-210 缺氧诱导因子-1Α 

分 类 号：R-332[医药卫生]