真孕受体用于金黄仓鼠胚胎移植的方法建立与优化  

Establishment and optimization of a method for transferring golden hamster embryos using true pregnancy recipients

作　　者：赖娅娜 曾文滔 LAI Yana;ZENG Wentao(Animal Core Facility of Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]南京医科大学医药实验动物中心,南京211166

出　　处：《中国比较医学杂志》2024年第10期72-77,共6页Chinese Journal of Comparative Medicine

基　　金：江苏省实验动物协会科研项目(DWXH202303);江苏省卫健委医学科研项目(Z2023003);国家重点研发计划(2021YFF0702500)。

摘　　要：目的 优化金黄仓鼠胚胎移植技术,探索金黄仓鼠真孕受体最佳移植胚胎数目及发育时期。方法 建立真孕白化金黄仓鼠种群作为胚胎移植受体,比较胚胎发育期、受体减胚及二次移植受体对产仔率、供胚得仔率及后代存活率的影响,确定真孕受体最佳胚胎移植枚数及移植方法。结果 相对于野生型金黄仓鼠作为移植受体,真孕白化受体可快速判定子代来源,并适用于多种以胚胎移植为基础的生殖类实验方案,采用受精卵或二细胞胚胎对移植效果无显著影响(P>0.05);减胚组能显著提高供胚得仔率(22%,P<0.05),二次移植受体的未孕率显著提高(42%,P<0.01);移植6~10枚胚胎的供胚得仔率最高(27%,P<0.01),存活率正常(89%)。结论 6~10枚供体胚胎有助于提高供胚得仔率和存活率,基于白化真孕受体的胚胎移植方法,为金黄仓鼠的应用与开发提供了技术支撑。Objective To optimize the technical parameters used for embryo transfer in golden hamsters,and explore the optimal number and developmental period of transplanted embryos for golden hamster pregnancy recipients.Methods We established a population of true pregnant albino golden hamster embryo transfer recipients and compared the effects of different embryonic developmental stages,recipient embryo reduction,and secondary transfer recipients on litter yield,donor embryo yield,and offspring survival rate.Results Compared with wild-type golden hamster transplant recipients,true pregnancy albino recipients allowed the origin of the offspring to be determined quickly,and were suitable for various reproductive experimental programs based on embryo transfer.The use of fertilized eggs or two-cell embryos had no significant impact on the transfer effect(P>0.05);however,the rate of donor embryos was significantly increased in the recipient embryo-reduction group(22%,P<0.05).The second transfer recipient's non-pregnancy rate was significantly increased(42%,P<0.01).The highest embryo yield rate(27%,P<0.01)and normal survival rate(89%)occurred with the transfer of 6~10 embryos.Conclusions The transfer of 6~10 donor embryos may improve the yield and survival rate of donor embryos.Here,we successfully established an embryo transfer method using pregnant albino golden hamsters as recipients,thus providing technical support for the application and development of gene modification models in golden hamsters.

关 键 词：金黄仓鼠 受精卵 二细胞胚胎 胚胎移植 

分 类 号：R-33[医药卫生]