上调Cx43蛋白表达增强自杀基因治疗鼻咽癌的旁观者效应  

Up-regulation of CX43 expression enhances the Bystander effect of suicide gene therapy for nasopharyngeal carcinoma

作　　者：曾友根 夏胜岚 张燕红 李宝金 ZENG You-Gen;XIA Sheng-Lan;ZHANG Yan-Hong(Department of Otorhinolaryngology,Medical college,Jinggangshan university,Ji’an 343000,Jiangxi,China)

机构地区：[1]井冈山大学附属医院耳鼻喉科,江西吉安343000 [2]广州医科大学附属市八医院

出　　处：《吉林医学》2024年第11期2609-2612,共4页Jilin Medical Journal

基　　金：江西省教育厅科技计划项目[项目编号:GJJ2201613];江西省卫健委科技计划项目[项目编号:SKJP220229759]。

摘　　要：目的:研究上调Cx43基因蛋白表达增强自杀基因治疗鼻咽癌的旁观者效应。方法:构建带Cx43基因的重组质粒,转染鼻咽癌细胞CNE-2和CNE-2/tk,实验分组:Cx43未转染组:不同比例鼻咽癌CNE-2细胞与CNE-2tk细胞混合,Cx43转染组:不同比例鼻咽癌CNE-2-Cx43细胞与CNE-2tk-Cx43细胞混合;对照分组:对照组1:不同比例鼻咽癌CNE-2细胞与CNE-2tk细胞混合;对照组2:不同比例鼻咽癌CNE-2-Cx43细胞与CNE-2tk-Cx43细胞混合。以不同对照组比较Cx43转染组与未转染组细胞存活率的差异。结果:以对照组1作为对照,tk^(-)与tk^(+)细胞的混合比例分别为100∶0,95∶5,90∶10,80∶20,75∶25,50∶50,0∶100时,Cx43未转染组与Cx43转染组细胞存活率分别对应为94.3%∶82.4%,83.2%∶63.5%,73.3%∶51.6%,52.7%∶33.9%,38.2%∶27.6%,17.6%∶16.8%,8.1%∶6.3%。tk^(-)与tk^(+)细胞的混合比例分别为100∶0,95∶5,90∶10,80∶20,75∶25时,两组比较差异有统计学意义(P<0.05),其他各组比较,差异无统计学意义(P>0.05);以对照组1作为Cx43未转染组对照,以对照组2作为Cx43转染组对照,tk^(-)与tk^(+)细胞的混合比例分别为100∶0,95∶5,90∶10,80∶20,75∶25,50∶50,0∶100时,Cx43未转染组与Cx43转染组细胞存活率分别对应为91.8%∶94.7%,82.1%∶71.9%,72.6%∶58.1%,51.6%∶38.7%,35.5%∶29.8%,13.2%∶16.8%,7.6%∶6.3%。tk^(-)与tk^(+)细胞的混合比例分别为95∶5,90∶10,80∶20,75∶25时,两组比较,差异有统计学意义(P<0.05),其他各组比较,差异无统计学意义(P>0.05)。结论:上调鼻咽癌细胞的Cx43和缝隙连接可明显增强自杀基因治疗鼻咽癌旁观者杀伤效应,为鼻咽癌的防治开拓新的方法和思路。Objective To study the Bystander effect of upping CX43 expression to enhance suicide gene therapy for nasopharyngeal carcinoma.Method The recombinant plasmids carrying CX43 gene were constructed and transfected into nasopharyngeal carcinoma cells CNE-2 and CNE-2/TK,the difference of cell survival rate between CX43 non-transfected group and CX43 transfected group was compared,and the CNE-2/CNE-2 TK mixed cells(control group 1)was used as the control of CX43 non-transfected group,cNE-2-CX43/CNE-2 tk-Cx43 mixed cells(control group 2)were used as the control of CX43 transfected group.The difference of cell survival rate between CX43 non-transfected group and CX43 transfected group was compared.Results Control group 1 as control,the mixed ratios of tk^(-)and tk^(+)cells were 100∶0,95∶5,90∶10,80∶20,75∶25,50∶50,0∶100,respectively,the cell survival rates of CX43 non-transfected group and CX43 transfected group were 94.3%∶82.4%,83.2%∶63.5%,73.3%∶51.6%,52.7%∶33.9%,38.2%∶27.6%,17.6%∶16.8%,8.1%∶6.3%,respectively.When the mixture ratio of tk^(-)and tk^(+)cells was 100.0,95∶5,90∶10,80∶20,75∶25 respectively,there was significant difference between the two groups(P<0.05),while there was no difference in other groups(P>0.05)CNE-2/CNE-2 TK mixed cells(control group 1)were used as the control of CX43 non-transfected group,and CNE-2/CNE-2 TK mixed cells(control group 1)were used as the control of CX43 non-transfected group,when the mixture ratio of tk^(-)and tk^(+)cells was 100∶0,95∶5,90∶10,80∶20,75∶25,50∶50,0∶100,the cell survival rates of CX43 non-transfected group and CX43 transfected group were 91.8%∶94.7%,respectively,82.1%∶71.9%,72.6%∶58.1%,51.6%∶38.7%,35.5%∶29.8%,13.2%∶16.8%,7.6%∶6.3%.When the mixture ratio of tk^(-)and tk^(+)cells was 95∶5,90∶10,80∶20,75∶25 respectively,there was significant difference between the two groups(P<0.05),while there was no difference in other groups(P>0.05).Conclusion The up-regulation of Cx43 and gap junction in NPC cells co

关 键 词：Cx43基因蛋白 单纯疱疹病毒胸苷激酶 旁观者效应 鼻咽癌 

分 类 号：R739.63[医药卫生—肿瘤]