鼻咽癌组织中核连蛋白2、整合素β3表达及其临床预后评估价值  

A study on the expression and prognostic value of nucleonectin 2 and integrinβ3 in nasopharyngeal cancer tissues

作　　者：徐菲 黄庆艳 周江会 黄佳露 翟亮 Xu Fei;Huang Qingyan;Zhou Jianghui;Huang Jialu;Zhai Liang(Department of Otolaryngology,First Affiliated Hospital of Naval Medical University,Shanghai 200433,China;不详)

机构地区：[1]海军军医大学第一附属医院耳鼻喉科,上海200433 [2]上海中医药大学附属宝山医院耳鼻喉科,201999

出　　处：《疑难病杂志》2024年第11期1373-1378,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：上海市卫生健康委员会卫生行业临床研究项目(202040168)。

摘　　要：目的研究鼻咽癌(NPC)组织中核连蛋白2(NUCB2)、整合素β3(ITGB3)的表达及其对NPC患者预后评估价值。方法回顾性收集2017年3月—2018年3月海军军医大学第一附属医院耳鼻喉科收治的NPC术后患者94例的癌组织及癌旁组织。采用实时荧光定量PCR(qPCR)和免疫组织化学法检测癌组织和癌旁组织中NUCB2、ITGB3 mRNA和蛋白表达;Kaplan-Meier法分析NUCB2、ITGB3 mRNA表达对NPC患者生存预后的影响;Cox回归分析NPC患者预后的影响因素。结果NPC患者癌组织NUCB2、ITGB3蛋白表达阳性率分别为95.74%(90/94)、87.23%(82/94),高于癌旁组织的8.51%(8/94)、10.64%(10/94),差异有统计学意义(χ^(2)/P=143.323/<0.001、29.139/<0.001);NPC患者癌组织中NUCB2、ITGB3 mRNA的相对表达量高于癌旁组织,差异有统计学意义(t/P=49.760/<0.001,43.704/<0.001);TNM分期Ⅲ~Ⅳ期的NPC癌组织中NUCB2、ITGB3 mRNA表达水平高于TNM分期Ⅰ~Ⅱ期(t/P=17.443/<0.001,14.275/<0.001)。NUCB2高表达组5年总生存率为52.17%(24/46),低于NUCB2低表达组的83.33%(40/48)(Log-rankχ^(2)=10.941,P=0.001);ITGB3高表达组5年总生存率为53.33%(24/45),低于ITGB3低表达组的81.63%(40/49)(Log-rankχ^(2)=9.832,P=0.002)。Cox回归分析结果表明,TNM分期Ⅲ~Ⅳ期、NUCB2 mRNA升高、ITGB3 mRNA升高为影响NPC患者预后的独立危险因素[OR(95%CI)=1.608(1.225~2.112),1.839(1.228~2.753),1.738(1.246~2.426)]。结论NPC组织中NUCB2、ITGB3 mRNA和蛋白表达上调,两者参与NPC肿瘤的进展过程,是评估NPC患者预后的标志物。Objective To study the expression of nucleolin 2(NUCB2)and integrinβ3(ITGB3)in nasopharyngeal carcinoma(NPC)and their prognostic value in NPC patients.Methods The cancer tissues and adjacent tissues of 94 patients with NPC who were admitted to the Department of Otolaryngology in the First Affiliated Hospital of Naval Medical University from March 2017 to March 2018 were collected.Real-time fluorescence quantitative PCR(qPCR)and immunohistochemistry were used to detect the expression of NUCB2 and ITGB3 mRNA and protein in cancer tissues and adjacent tissues.Kaplan-Meier method was used to analyze the effect of NUCB2 and ITGB3 mRNA expression on the survival and prognosis of NPC patients.Cox regression analysis was used to analyze the prognostic factors of NPC patients.Results The positive rates of NUCB2 and ITGB3 protein expression in cancer tissues of NPC patients were 95.74%(90/94)and 87.23%(82/94),respectively,which were higher than 8.51%(8/94)and 10.64%(10/94)in adjacent tissues(χ^(2)/P=143.323/<0.001,29.139/<0.001);The relative expression of NUCB2 and ITGB3 mRNA in cancer tissues of NPC patients was higher than that in adjacent tissues,and the difference was statistically significant(t/P=49.760/<0.001,43.704/<0.001);The expression levels of NUCB2 and ITGB3 mRNA in NPC tissues of TNM stageⅢ~Ⅳwere higher than those of TNM stageⅠ~Ⅱ(t/P=17.443/<0.001,14.275/<0.001).The 5-year overall survival rate of NUCB2 high expression group was 52.17%(24/46),which was lower than that of NUCB2 low expression group(83.33%,40/48)(Log-rankχ^(2)=10.941,P=0.001);The 5-year overall survival rate of ITGB3 high expression group was 53.33%(24/45),which was lower than 81.63%(40/49)of ITGB3 low expression group(Log-rankχ^(2)=9.832,P=0.002).TNM stageⅢ~IV,increased NUCB2 mRNA and increased ITGB3 mRNA were independent risk factors affecting the prognosis of NPC patients[OR(95%CI)=1.608(1.225-2.112),1.839(1.228-2.753),1.738(1.246-2.426)].Conclusion The mRNA and protein expressions of NUCB2 and ITGB3 are up-regulated in NPC tiss

关 键 词：鼻咽癌 核连蛋白2 整合素Β3 临床病理特征 预后 

分 类 号：R739.6[医药卫生—肿瘤]