油莎豆种质资源光合特性分析  

Analysis on photosynthetic characteristics of Cyperus esculentus L.germplasm resources

作　　者：李梁 叶君 李娟[1,3] 路战远[1,2,3] 李存东 刘文景[1] 张德健 赵小庆[1,2] 王登云 段锐 史学芬 赵贤 LI Liang;YE Jun;LI Juan;LU Zhanyuan;LI Cundong;LIU Wenjing;ZHANG Dejian;ZHAO Xiaoqing;WANG Dengyun;DUAN Rui;SHI Xuefen;ZHAO Xian(School of Life Sciences,Inner Mongolia University,Hohhot 010021,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;College of Agronomy,Hebei Agricultural University,Baoding 071001,China;Dengkou County Agricultural and Animal Husbandry Technology Extension Center,Dengkou 015200,China;Ordos Agricultural and Animal Husbandry Science Research Institute,Ordos 017000,China;Bayannur Modern Animal Husbandry Business Development Center,Bayannur 015000,China)

机构地区：[1]内蒙古大学生命科学学院,内蒙古呼和浩特010021 [2]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031 [3]河北农业大学农学院,河北保定071001 [4]磴口县农牧业技术推广中心,内蒙古磴口015200 [5]鄂尔多斯市农牧业科学研究院,内蒙古鄂尔多斯017000 [6]巴彦淖尔市现代农牧事业发展中心,内蒙古巴彦淖尔015000

出　　处：《江苏农业学报》2024年第9期1594-1606,共13页Jiangsu Journal of Agricultural Sciences

基　　金：内蒙古“草原英才”工程领军人才项目;内蒙古自治区2022年科技领军人才团队项目(2022LJRC0010);内蒙古农牧业创新基金项目(2022CXJJN05);内蒙古自治区自然科学基金项目(2024MS03007)。

摘　　要：为鉴选油莎豆高光效优异种质,以40份油莎豆种质资源为试验材料,测定油莎豆叶片气体交换参数、叶绿素荧光参数以及叶绿素含量等光合参数,利用相关性分析和主成分分析等多种统计学方法,综合评价与分析油莎豆种质资源光合特性。结果表明,20个光合参数的变异系数为1.23%~48.51%,总性能指数(PI_(total),光系统Ⅱ、光系统Ⅰ和系统间电子传递链的整体功能活性)的变异系数最大。PI_(total)与F_(v)/F_(m)(t=0时,原初光化学反应最大量子产率)、S_(m)(受体库容量)、RE_(o)/RC(单个活性反应中心传递至光合系统Ⅰ末端电子受体使其还原的电子通量)、psi(E_(o))[开始照明时,由活性反应中心捕获的单个激子驱动除还原辅酶A(QA)外的电子传递的效率]、phi(E_(o))(开始照明时,还原辅酶A至除还原辅酶A外的电子传递链电子受体电子传递的量子效率)、delta(R_(o))(开始照明时,由活性反应中心捕获的单个激子驱动单个电子由QA经电子传递链至PSⅠ受体侧末端电子受体的效率)、phi(R_(o))(光系统Ⅰ受体侧末端电子受体还原的量子效率)、RE_(o)/CS_(m)(t=t F_(m),单个活性反应中心传递至PSI末端电子受体使其还原的能量通量)、PI abs(以吸收光能为基础的性能参数)呈现显著正相关,与ABS/RC(单位反应中心吸收的光能)、DI_(o)/RC(单个活性反应中心耗散的总能量)、TR_(o)/RC(单位反应中心捕获的用于还原辅酶A的能量)呈现极显著负相关。主成分最小数据组分析得出,PI_(total)、ET_(o)/RC(单位反应中心传递的能量)、ET_(o)/CS_(m)(达到荧光测量最大值时,单位叶面积用于电子传递的能量)、净光合速率(P_(n))和气孔导度(G_(s))可作为油莎豆高光效种质资源鉴选的重要指标。聚类分析结果表明,40份种质资源可划分为3类。其中,第Ⅰ类群有16份种质,主要表现为P_(n)、G_(s)显著高于其他2个类群。第Ⅱ类群有13份种质,主要表现�In order to select excellent germplasm with high photosynthetic efficiency from Cyperus esculentus L.,40 C.esculentus L.germplasm resources were used as experimental materials to measure photosynthetic parameters such as gas exchange parameters,chlorophyll fluorescence parameters,and chlorophyll content in C.esculentus L.leaves.Multiple statistical methods such as correlation analysis and principal component analysis were used to comprehensively evaluate and analyze the photosynthetic characteristics of C.esculentus L.germplasm resources.The results showed that the coefficients of variation of 20 photosynthetic parameters ranged from 1.23%to 48.51%.The highest coefficient of variation was found in PI_(total)(the overall functional activity of photosynthetic systemⅡ,photosynthetic systemⅠand electron transport chain between systems).PI_(total) was in significant positive correlation with F_(v)/F_(m)(when t=0,the maximum quantum yield of the initial photochemical reaction was obtained),S_(m)(receptor library capacity),RE_(o)/RC(the flux of electrons from a single active reaction center to the electron acceptor at theⅠend of the photosynthetic system for reduction),psi(E_(o))(efficiency of electron transfer driven by a single exciton,except for the reductive coenzyme A(QA)),phi(E_(o))(at the start of illumination,the quantum efficiency of electron transferred from reductive coenzyme A to the electron acceptors of the respiratory chain other than reductive enzyme A),delta(R_(o))(efficiency of a single exciton captured by an active reaction center to drive a single electron from the QA electron transport chain to the end of the electron acceptor on the PSI receptor side at the start of illumination),phi(R_(o))(quantum efficiency of electron acceptor reduction at the end of photosystemⅠreceptor),RE_(o)/CS_(m)(t=t F_(m),the energy flux from a single active reaction center to the electron acceptor at the end of the PSⅠto reduce it),PI abs(performance parameters based on absorption of light energy),and was in hi

关 键 词：油莎豆 种质资源 气体交换参数 叶绿素荧光参数 

分 类 号：S565.9[农业科学—作物学] Q945.11[生物学—植物学]