氧诱导视网膜病变模型小鼠视网膜组织中SHP2的表达及意义  

Expression and significance of SHP2 in retinal tissues of mice with oxygen-induced retinopathy

作　　者：陈锦 刘向玲 苏绍波 马高恩 CHEN Jin;LIU Xiangling;SU Shaobo;MA Gaoen(The Third Clinical College of Xinxiang Medical University,Xinxiang 453003,Henan Province,China;Department of Ophthalmology,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,Henan Province,China)

机构地区：[1]新乡医学院第三临床学院,河南新乡453003 [2]新乡医学院第三附属医院眼科,河南新乡453003

出　　处：《新乡医学院学报》2024年第11期1016-1021,共6页Journal of Xinxiang Medical University

基　　金：国家自然科学基金资助项目(编号:31471122);河南省医学科技攻关计划省部共建项目(编号:SBGJ202102190)。

摘　　要：目的探讨含Src同源-2结构域的蛋白酪氨酸磷酸酶2(SHP2)及磷酸化SHP2(P-SHP2)在氧诱导视网膜病变(OIR)组织中的表达及意义。方法将20只清洁级C57BL/6J(B6)7日龄新生小鼠随机分为正常对照组和OIR组,每组10只。正常对照组小鼠与哺乳母鼠共同置于常氧、室温正常环境中饲养。OIR组小鼠与哺乳母鼠共同置于温度22~25℃、湿度(60±10)%、氧气体积分数稳定于(75±5)%的氧箱中,持续5 d后转移至常氧环境中。分别于12、14、17日龄时取正常对照组和OIR组小鼠各3只,采用Western blot法检测2组小鼠视网膜组织中SHP2、P-SHP2蛋白表达。随机取正常对照组和OIR组17日龄小鼠各2只,采用苏木精-伊红染色法观察小鼠视网膜组织病理学情况。结果正常对照组17日龄小鼠视网膜组织各层细胞结构排列整齐,内界膜与内皮细胞核均完整,形态正常。OIR组17日龄小鼠视网膜组织各层细胞间结构紊乱,可见血管内皮细胞核突破视网膜内界膜。不同日龄正常对照组小鼠视网膜组织中SHP2蛋白相对表达量比较差异无统计学意义(F=2.052,P>0.05)。正常对照组小鼠14、17日龄时视网膜组织中P-SHP2蛋白相对表达量显著低于12日龄时(P<0.05),17日龄时视网膜组织中P-SHP2蛋白相对表达量显著低于14日龄时(P<0.05)。OIR组小鼠14日龄时视网膜组织中SHP2蛋白相对表达量显著高于12日龄和17日龄时(P<0.05);OIR组小鼠17日龄时视网膜组织中SHP2蛋白相对表达量与12日龄时比较差异无统计学意义(P>0.05)。OIR组小鼠14日龄时视网膜组织中P-SHP2蛋白相对表达量显著高于12日龄和17日龄时(P<0.05);OIR组小鼠17日龄时视网膜组织中P-SHP2蛋白相对表达量与12日龄时比较差异无统计学意义(P>0.05)。12、17日龄时,OIR组小鼠视网膜组织中SHP2蛋白相对表达量显著低于正常对照组(P<0.05);2组小鼠14日龄时视网膜组织中SHP2蛋白相对表达量比较差异无统计学意义(P>0.05)�Objective To investigate the expression and significance of Src homology 2 domain-containing protein tyrosine phosphatase 2(SHP2)and phosphorylated SHP2(P-SHP2)in oxygen-induced retinopathy(OIR).Methods Twenty clean-grade C57BL/6J(B6)7-day-old neonatal mice were randomly divided into normal control group and OIR group,with 10 mice in each group.The mice in the normal control group and the nursing mother mice were fed together in a normal oxygen and room temperature environment.Mice in the OIR group were placed in an oxygen chamber with a temperature of 22-25℃,humidity of(60±10)%and oxygen volume fraction stable at(75±5)%for five days together with the nursing mother mice,and then transferred to a normal oxygen environment.At 12,14,17 days of age,three mice were selected from the normal control group and the OIR group,respectively;and the expressions of SHP2 and P-SHP2 proteins in the retinal tissues of mice in the two groups were detected by Western blot.Two 17-day-old mice were randomly selected from the normal control group and the OIR group,respectively;and the retinal histopathology of mice was observed by hematoxylin-eosin staining.Results In the normal control group,the cell structure in all layers of the retinal tissue of 17-day-old mice was orderly,the inner limiting membrane and endothelial nucleus were intact,and the shape was normal.In the OIR group,the intercellular structure in all layers of the retinal tissue of 17-day-old mice was disordered,and the vascular endothelial nucleus was seen to break through the retinal inner limiting membrane.There was no statistically significant difference in the relative expression of SHP2 protein in the retinal tissues of mice at different ages in the normal control group(F=2.052,P>0.05).The relative expression level of P-SHP2 protein in the retinal tissues of mice at 14,17 days of age in the normal control group was significantly lower than that at 12 days of age(P<0.05),and the relative expression level of P-SHP2 protein in the retinal tissues of mice at 17 d

关 键 词：含Src同源-2结构域的蛋白酪氨酸磷酸酶2 磷酸化 缺氧性视网膜病变 

分 类 号：R774.1[医药卫生—眼科]