抗生素对高脂饮食诱导肥胖小鼠能量代谢及脂肪组织功能的影响  

Effects of antibiotics on energy metabolism and adipose tissue function in high-fat diet-induced obese mice

作　　者：崔远捷 毕馨文 卜凡 崔佳 陆秋娴 何方[1] 杨华[1] 李鸣[1] CUI Yuanjie;BI Xinwen;BU Fan;CUI Jia;LU Qiuxian;HE Fang;YANG Hua;LI Ming(West China School of Public Health/The Fourth Hospital of West China,Sichuan University,Chengdu 610041,China;Sichuan Academy of Medical Sciences·Sichuan Provincial People′s Hospital(Affiliated Hospital of UESTC),Chengdu 610072,China;Second People′s Hospital of Yibin,Yibin,Sichuan 644000,China)

机构地区：[1]四川大学华西公共卫生学院四川大学华西第四医院,四川成都610041 [2]四川省医学科学院四川省人民医院(电子科技大学附属医院),四川成都610072 [3]四川省宜宾市第二人民医院,四川宜宾644000

出　　处：《公共卫生与预防医学》2024年第6期6-10,共5页Journal of Public Health and Preventive Medicine

基　　金：国家自然科学基金(82073550)。

摘　　要：目的以抗生素及高脂饮食为干预手段,研究其对小鼠能量代谢、白色脂肪组织(WAT)及棕色脂肪组织(BAT)棕色化相关功能表达的影响,为脂肪组织在肥胖防治中的可能作用机制提供新思路。方法10周龄C57BL/6雄性小鼠80只,前期同在普通饲料喂养下,抗生素灌胃组(AG)和抗生素高脂组(AFG)以混合抗生素灌胃;空白组(BG)及高脂饮食组(FG)以生理盐水灌胃2周,结束灌胃操作后对FG组和AFG组给予高脂饲料进行肥胖造模、BG组及AG组继续以普通饲料喂养8周(N=20);实验结束后各组进行β3-肾上腺素能受体激动剂连续注射5d,所进食高脂/普通饲料保持不变。实验结束测量小鼠基础代谢率(BMR)、空腹血糖(FBG)及直肠温度,收集小鼠粪便、血液、皮下白色脂肪、附睾及肩胛区棕色脂肪组织。使用全自动生化分析仪测定血生化指标;逆转录聚合酶链式反应(RT-qPCR)法分别测量WAT与BAT脂肪组织棕色化相关基因表达;实时荧光定量聚合酶链式反应(qPCR)法测定WAT线粒体DNA(mt DNA)表达。结果从第4周起直至实验结束,AFG组体重显著高于AG组,且显著低于FG组(P<0.05)。AFG组体重、脏器系数、血清TC水平、直肠温度及WAT细胞直径均显著高于AG组;AFG组血清FBG、TC、LDL水平显著低于FG组、(P<0.05)。整体BMR(mlO2/h)FG组显著高于BG组,AFG组显著高于AG;单位体重BMR(mlO2/h/g)AFG显著高于FG组(P<0.05)。AFG组小鼠BAT的RIP140、PPAR-γ和UCP-1表达显著高于FG组,AFG组WAT的mt DNA拷贝数显著高于FG组(P<0.05)。结论抗生素干预能上调高脂饮食小鼠棕色脂肪相关基因表达提高棕色脂肪活性、增加白色脂肪线粒体相对拷贝数提高白色脂肪棕色化水平,促进机体产热,提高成年肥胖小鼠单位体重BMR进而提高机体整体能量代谢状况,一定程度上减缓单纯高脂饮食诱导的小鼠体重上升。Objective To study the effects of antibiotics and high-fat diet on energy metabolism and the browning of white adipose tissue(WAT)and brown adipose tissue(BAT)in mice,so as to provide new ideas for the possible mechanism of adipose tissue in the prevention and treatment of obesity.Methods A total of 8010-week-old C57BL/6 male mice were fed with normal diet in the early stage,and the antibiotic gavage group(AG)and antibiotic high-fat group(AFG)were given mixed antibiotics by gavage.The blank group(BG)and the high-fat diet group(FG)were given normal saline intragastric solution for 2 weeks,and after the gavage operation,the FG group and the AFG group were given high-fat diet for obesity modeling,and the BG group and AG group continued to be fed with normal diet for 8 weeks(N=20).After the experiment,each group was injected withβ3-adrenergic receptor agonists for 5 days,and the high-fat/ordinary diet remained unchanged.At the end of the experiment,basal metabolic rate(BMR),fasting blood glucose(FBG)and rectal temperature were measured,and feces,blood,subcutaneous white fat,epididymis and brown adipose tissue in the scapular area of mice were collected.The automatic biochemical analyzer was used to determine the blood biochemical indexes;reverse transcription polymerase chain reaction(RT-qPCR)was used to measure the expression of genes related to browning of WAT and BAT adipose tissue,respectively.Real-time quantitative polymerase chain reaction(qPCR)was used to determine the expression of WAT mitochondrial DNA(mt DNA).Results From the 4th week to the end of the experiment,the weight of the AFG group was significantly higher than that of the AG group and significantly lower than that of the FG group(P<0.05).The body weight,organ coefficient,serum TC level,rectal temperature and WAT cell diameter in the AFG group were significantly higher than those in the AG group.The serum levels of FBG,TC and LDL in the AFG group were significantly lower than those in the FG group(P<0.05).The overall BMR(mlO2/h)FG group was signif

关 键 词：肥胖 能量代谢 白色脂肪 棕色脂肪 

分 类 号：R181[医药卫生—流行病学]