楠竹茎水浸提物抗氧化活性及对t-BHP诱导Caco-2细胞氧化损伤的保护作用  

Antioxidant activity of water extract from bamboo stems and its protective effect on t-BHP induced oxidative damage in Caco-2 cells

作　　者：袁欣 黄云龙 解晓敏 钟子涵 陈佳蕊 鲍翠玉 杨旭 马萍 YUAN Xin;HUANG Yunlong;XIE Xiaomeng;ZHONG Zihan;CHEN Sirui;BAO Cuiyu;YANG Xu;MA Ping(School of Pharmacy,Hubei University of Science and Technology,Xianning,Hubei 437100,China;Hubei Industrial Technology Research Institute of Intelligent Health,Xianning,Hubei 437100,China;School of Basic Medical Sciences,Hubei University of Science and Technology,Xianning,Hubei 437100,China;Institute of Natural Antioxidants and Anti-inflammation,Dali University,Dali,Yunnan 671003,China)

机构地区：[1]湖北科技学院药学院,湖北咸宁437100 [2]湖北省智慧康养产业技术研究院,湖北咸宁437100 [3]湖北科技学院基础医学院,湖北咸宁437100 [4]大理大学天然抗氧化剂和抗氧化炎症研究院,云南大理671003

出　　处：《公共卫生与预防医学》2024年第6期50-54,共5页Journal of Public Health and Preventive Medicine

基　　金：咸宁市科技计划社发类研发重点专项项目(2021SFYF007,2023SFYF095);湖北科技学院大学生创新创业重点孵化培育项目(2023019),湖北科技学院科研创新团队项目(2023T08)。

摘　　要：目的探究楠竹茎提取物的抗氧化活性,并研究楠竹茎水浸提物对叔丁基过氧化氢(t-BHP)诱导的人结肠腺癌细胞(Caco-2)氧化炎症的治疗作用。方法用ABTS、DPPH、FRAP法测定楠竹茎乙酸乙酯提取物、石油醚提取物、95%乙醇提取物、正丁醇提取物及蒸馏水提取物的细胞外抗氧化活性,以人肠Caco-2细胞为模型细胞,选用t-BHP为氧化应激造模剂。CCK-8实验用于检测细胞活力,MDA、8-OHdG、TNF-α和IL-1β用于测定抗氧化应激效果。结果楠竹的五种提取物均有一定的抗氧化活性,其中楠竹茎水浸提取物的抗氧化能力最佳,能显著降t-BHP诱导Caco-2产生的氧化应激分子生物和炎症因子的含量。结论咸宁市楠竹茎具有较强的抗氧化活性,其有望成为防治氧化应激的重要物质,可用于抗氧化产品的新型研发等。Objective To investigate the antioxidant activity of bamboo stem extracts and the therapeutic effect of bamboo stem water extract on oxidative inflammation induced by tert butyl hydroperoxide(t-BHP)in human colon adenocarcinoma cells(Caco-2).Methods In this study,ABTS,DPPH,and FRAP assays were used to determine the extracellular antioxidant activity of petroleum ether extract,ethyl acetate extract,n-butanol extract,95%ethanol extract,and distilled water extract from bamboo stems.The human intestinal Caco-2 cell line was used as the model cell,and t-BHP was selected as the oxidative stress modeling agent.The CCK-8 assay was used to detect cell viability and the optimal oxidative damage concentration of t-BHP.The content of MDA,8-OHdG,TNF-αand IL-1βwere detected to assess antioxidant stress effect.Results The five extracts of bamboo all had certain antioxidant activity,among which the water extract of bamboo stem had the best comprehensive antioxidant activity with high cell viability in Caco-2 cells.The optimal modeling concentration of t-BHP was 200μMol/L.The water extract of bamboo stem significantly reduced the content of oxidative stress related biomarkers and inflammatory factors in Caco-2 cells induced by t-BHP.Conclusion The stem extracts of bamboo in Xianning City have strong in vitro antioxidant activity.Among them,the water extract of bamboo stem has a protective effect on t-BHP induced oxidative damage in Caco-2 cells,suggesting that the water extract possesses a potential to be developed as new antioxidant products for clinical prevention and treatment of oxidative damage related diseases.

关 键 词：楠竹茎 自由基清除率 Caco-2细胞 叔丁基过氧化氢 抗氧化活性 

分 类 号：R181[医药卫生—流行病学]