MgH_(2)复合明胶海绵的制备及抑制变形链球菌的作用研究  

Stydy on the preparation of MgH2 composite gelatin sponge and its effect on inhibiting Streptococcus mutans

作　　者：季丽娜 张骞骞 刘昕[1] 陆华[1] Ji Lina;Zhang Qianqian;Liu Xin;Lu Hua(Department of Dental Materials,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology,Shanghai 200011)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔材料科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海200011

出　　处：《口腔材料器械杂志》2024年第4期208-213,共6页Chinese Journal of Dental Materials and Devices

基　　金：国家自然科学基金项目(82271024);上海交通大学医学院附属第九人民医院交叉基金(JYJC202309);上海市重中之重研究中心项目(2022ZZ01017);中国医学科学院医学与健康科技创新工程项目(2019-I2M-5-037)。

摘　　要：目的通过将MgH_(2)微粒与临床常用的明胶海绵复合,制备MgH_(2)微粒复合明胶海绵研究其抗变形链球菌作用。方法使用硅烷偶联剂将氢化镁微粒分散在明胶海绵表面,并分别制备出MgH_(2)和明胶海绵质量比例为1∶8和1∶4的MgH_(2)复合明胶海绵。利用扫描电子显微镜观察MgH_(2)微米颗粒复合明胶海绵的微观结构和微粒分布。通过平板涂布实验,评估不同浓度MgH_(2)复合明胶海绵对变形链球菌的抑制效果。通过噻唑蓝(MTT)比色法评估MgH_(2)微粒和MgH_(2)复合明胶海绵与成纤维细胞共培养后的细胞存活率。结果成功制备了MgH_(2)复合明胶海绵,MgH_(2)微粒均匀分布在胶原海绵的表面且保持原有形貌。当MgH_(2)复合明胶海绵混合比例为1∶4时,在体外条件下具有潜在的抗变形链球菌性能。此外,MgH_(2)复合明胶海绵的细胞存活率为(72.02±3.89)%,与胶质银海绵组[(2.42±0.13)%]相比,差异具有统计学意义(P<0.01)。但是当MgH_(2)微粒浓度超过2 mg/mL时表现出较强的细胞毒性,细胞存活率为(1.11±0.17)%。结论MgH_(2)复合明胶海绵与胶质银明胶海绵相比具有较好的体外抗变形链球菌性能和细胞活力,有一定的临床应用潜能。Objective To synthesize an Magnesium hydride(MgH_(2))micro-particles composite gelatin sponge by incorporating MgH_(2)micro-particles into a clinically used gelatin sponges,and study its anti-Streptococcus mutans effect.Methods MgH_(2)particles were dispersed on the surface of gelatin sponge using a silane coupling agent,and MgH_(2)composite gelatin sponges were prepared with a mass ratio of MgH_(2)to gelatin sponge of 1:8 and 1:4,respectively.The microstructure and particle distribution of the MgH_(2)microparticles-gelatin sponge composites were observed using scanning electron microscopy.The inhibition effect of MgH_(2)composite gelatin sponges with different concentrations on Streptococcus mutans was evaluated through spread plate method.The cell viability of fibroblasts co-cultured with MgH_(2)micro-particles and MgH_(2)composite gelatin sponges using the MTT assay.Results MgH_(2)composite gelatin sponges were successfully prepared,with MgH_(2)particles uniformly distributed on the surface of the gelatin sponges and maintained the original morphology.The MgH_(2)composite gelatin with a mixing ratio of 1∶4 showed potential against Streptococcus mutans under in vitro conditions.Additionally,the cell viability of the MgH_(2)composite gelatin sponges was(72.02±3.89)%,which was statisti‐cally significantly different from the colloidal silver gelatin sponge group[(2.42±0.13)%].However,MgH_(2)micro-particles showed strong cytotoxicity at concentrations above 2 mg/mL,as indicated by the cell viability of(1.11±0.17)%.Conclusion MgH_(2)composite gelatin sponges exhibit enhanced anti-Streptococcus mutans activ‐ity and an acceptable cell viability compared to colloidal silver gelatin sponge,showing the potential for clinical ap‐plications.

关 键 词：氢化镁微粒 明胶海绵 抗菌 变形链球菌 

分 类 号：R783.1[医药卫生—口腔医学] TB332[医药卫生—临床医学]