油莎豆愈伤组织诱导及再生体系的建立  

Establishment of Callus Induction and Regeneration System of Cyperus esculentus

作　　者：朱雅婧 张向歌 许欣然[1] 胡海燕[1] 程珊 陈晨 李春鑫[1] 宋万献 宋雯栩 王会伟[1] ZHU Yajing;ZHANG Xiangge;XU Xinran;HU Haiyan;CHENG Shan;CHEN Chen;LI Chunxin;SONG Wanxian;SONG Wenxu;WANG Huiwei(Industrial Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院经济作物研究所,河南郑州450002

出　　处：《河南农业科学》2024年第10期73-81,共9页Journal of Henan Agricultural Sciences

基　　金：河南省重大科技专项(211100110100);河南省重点研发与推广专项(科技攻关)(232102110261,242102110290,242102110261)。

摘　　要：为建立高效的油莎豆愈伤组织诱导及再生体系,对适用于愈伤组织诱导的外植体(幼根、幼叶、幼叶鞘和分蘖节)进行筛选,并探讨不同植物生长调节剂组合对愈伤组织诱导、增殖和分化的影响。结果表明,油莎豆愈伤组织诱导的最佳外植体为分蘖节;最优愈伤组织诱导的植物生长调节剂组合为2.5 mg/L 2,4-D+0.6 mg/L 6-BA+0.6 mg/L NAA+0.9 mg/L ZT,诱导率可达65.58%;最优愈伤组织增殖的植物生长调节剂组合为0.5 mg/L 2,4-D+0.9 mg/L 6-BA+1.2 mg/L NAA+1.2 mg/L ZT,增殖率可达68.12%;最优愈伤组织分化的植物生长调节剂组合为0.9 mg/L 6-BA+0.6 mg/L NAA,分化率可达66.67%。试验筛选出了适合油莎豆愈伤组织诱导的最佳外植体,并优化了油莎豆愈伤组织诱导、增殖和分化的条件,为后续油莎豆体细胞杂交、遗传转化等生物技术的研究及育种应用奠定基础。In order to establish an efficient callus induction and regeneration system of Cyperus esculentus,the explants(young root,young leaf,young leaf sheath and tillering node)for callus induction were screened,and the effects of different plant growth regulator combinations on callus induction,proliferation and differentiation were explored.The results showed that the best explant for callus induction of Cyperus esculentus was tillering node;the optimal plant growth regulator combination for callus induction was 2.5 mg/L 2,4‑D+0.6 mg/L 6‑BA+0.6 mg/L NAA+0.9 mg/L ZT,and the induction rate could reach 65.58%;the optimal plant growth regulator combination for callus proliferation was 0.5 mg/L 2,4‑D+0.9 mg/L 6‑BA+1.2 mg/L NAA+1.2 mg/L ZT,and the proliferation rate could reach 68.12%;the optimal plant growth regulator combination for callus differentiation was 0.9 mg/L 6‑BA+0.6 mg/L NAA,and the differentiation rate could reach 66.67%.In this study,the best explants suitable for callus induction of Cyperus esculentus were screened,and the conditions of callus induction,proliferation and differentiation of Cyperus esculentus were optimized,which could lay a foundation for the subsequent research and breeding application of biotechnology such as somatic hybridization and genetic transformation of Cyperus esculentus.

关 键 词：油莎豆 愈伤组织 外植体 植物生长调节剂 再生体系 

分 类 号：S565.9[农业科学—作物学]