Single-cell transcriptomic profiling reveals ZEB1-mediated regulation in microglial subtypes and the impact of exercise on neuroinflammatory responses  

作　　者：Jin-Fang Wu Yi-Sheng Chen Yu-Chun Xie 

机构地区：[1]Department of Rehabilitation Medicine,Jiangsu Province Geriatric Hospital,Nanjing 210024,China [2]Fujian Key Laboratory of Toxicant and Drug Toxicology,Medical College,Ningde Normal University,Ningde 352100,China [3]Central Laboratory,Jiangsu Province Geriatric Hospital,Nanjing 210024,China

出　　处：《Traditional Medicine Research》2025年第2期55-64,共10页TMR传统医学研究

摘　　要：Background:This study aims to identify distinct cellular subtypes within brain tissue using single-cell transcriptomic analysis,focusing on specific biomarkers that differentiate cell types and the effects of traditional and exercise therapy.Methods:Four samples were analyzed:older control(OC),older exercise(OE),younger control(YC),and younger exercise(YE).Single-cell RNA sequencing was used to distinguish cellular subtypes through their biomarker profiles.Data visualization included violin and t-SNE plots to illustrate biomarker expression across cell clusters such as oligodendrocytes,microglia,and astrocytes.Additionally,BV2 cells were exposed to amyloid-beta fragments to simulate Alzheimer’s disease,assessing the impact of exercise-induced cellular responses.Results:Distinct cellular subtypes were identified:oligodendrocytes(MBP,St18),microglia(Dock8),and astrocytes(Aqp4,Gpc5).Sample OE was predominantly oligodendrocytes,while YE had more astrocytes,inhibitory neurons,and Canal-Retzius cells.YC showed a significant presence of Olfm3+ganglion neurons.ZEB1 gene knockout revealed changes in SMAD family gene expression,which regulate ferroptosis.Oxidative stress levels were also evaluated.Conclusion:This profiling enhances our understanding of brain cellular functions and interactions,potentially informing targeted therapies in neurological research.Exercise may influence brain cell immune responses and cell death pathways by regulating specific gene expressions,offering new insights for treating neuroinflammation and degeneration.

关 键 词：single-cell transcriptomics brain tissue cellular subtypes biomarker expression neuronal diversity 

分 类 号：R74[医药卫生—神经病学与精神病学]