基于网络药理学的刺五加抗帕金森病的作用机制研究  

Mechanism on anti-Parkinson's disease activity of Eleutherococcus senticosus based on network pharmacology

作　　者：宋鑫 寇学坤 李畅 张峻 李玉凤[2] 龙月红 邢朝斌 SONG Xin;KOU Xuekun;LI Chang;ZHANG Jun;LI Yufeng;LONG Yuehong;XING Zhaobin(College of Life Sciences,North China University of Science and Technology,Tangshan 063210,China;Department of Central Laboratory,Tangshan People's Hospital,Tangshan 063000,China)

机构地区：[1]华北理工大学生命科学学院,河北唐山063210 [2]唐山市人民医院中心实验室,河北唐山063000

出　　处：《邵阳学院学报（自然科学版）》2024年第5期68-76,共9页Journal of Shaoyang University：Natural Science Edition

摘　　要：目的基于网络药理学与分子对接方法探究刺五加治疗帕金森病(Parkinson's disease,PD)的作用机制。方法检索ETCM数据库获取并筛选刺五加活性成分,通过Swiss Target Prediction平台预测活性成分对应靶标,构建“刺五加-活性成分-靶点”网络图;检索GeneCards、DisGeNET数据库获取PD相关基因靶点,针对其与刺五加交集靶点,根据度值、中介中心性和接近中心性筛选核心靶点;使用STRING数据库构建PPI网络图,并进行GO与KEGG富集分析;最后使用AutoDock Vina进行分子对接。结果共检索出61种刺五加活性成分,确定了652个潜在靶点,506个可能适合治疗PD的潜在疾病靶点。分子对接结果表明,刺五加苷B、槲皮素和β-谷甾醇是刺五加治疗PD的核心活性成分,可分别作用于排名前3位的核心靶点GAPDH、AKT1和TNF。结论推测刺五加可能主要以神经活性配体-受体相互作用及cAMP信号通路来发挥治疗PD的作用。Objective To investigate the mechanism and molecular targets of Eleutherococcus senticosus in the treatment of Parkinson's disease(PD).Methods The active ingredients of Eleutherococcus senticosus were obtained and screened from the ETCM database,and their corresponding targets were predicted using the Swiss Target Prediction platform,where the network diagram of“E.senticosus-active compound-gene target”was constructed.The gene targets of PD were obtained from GeneCards and DisGeNET,and their intersection with the E.senticosus gene targets was screened based on degree value,mediation centrality,and proximity centrality.STRING database was used to construct a network diagram of PPI,followed by the enrichment analysis of GO and KEGG.Finally,molecular docking was performed using AutoDock Vina.Results A total of 652 of 61 bioactive compounds in E.senticosus were retrieved,and 652 potential targets were identified,including 506 potential disease targets probabely suitable for treating PD.The results of molecular docking show that eleutheroside B,quercetin,and β-sitosterol were the core active components of E.senticosus in the treatment of PD,and they could act on GAPDH,AKT1,and TNF,respectively.Conclusion It is speculated that E.senticosus may play a role in improving the treatment of PD mainly through the interaction of neuroactive ligand-receptor and cAMP signaling pathway.

关 键 词：刺五加 帕金森病 网络药理学 分子对接 信号通路 

分 类 号：R285[医药卫生—中药学]