不同粒色小麦籽粒中叶酸及衍生物含量分析  

Analysis of Folate Content and Its Derivatives in Grains of Wheat with Different Grain Colors

作　　者：田鹏 宋洁 李霞[2] 王裕智 武棒棒 TIAN Peng;SONG Jie;LI Xia;WANG Yuzhi;WU Bangbang(Shanxi Vocational University of Engineering Science and Technology,Shanxi Taiyuan 030619,China;Institute of Wheat Research,Shanxi Agricultural University,Shanxi Linfen 041000,China)

机构地区：[1]山西工程科技职业大学,太原030619 [2]山西农业大学小麦研究所,山西临汾041000

出　　处：《中国农业科技导报》2024年第11期56-65,共10页Journal of Agricultural Science and Technology

基　　金：山西省重点研发计划项目(2022ZDYF108);山西省哲学社会科学规划课题(2023YJ148)。

摘　　要：叶酸在促进细胞生长、维持细胞活性、参与细胞增殖和分裂过程中起关键作用。彩色小麦富含花青素、铁、锌、硒等功能营养物质,人们对彩色小麦需求越来越大,为研究彩色小麦籽粒叶酸及衍生物的含量,以42份不同粒色的小麦品种(系)为材料,利用高效液相色谱法测定了3个生长条件籽粒中四氢叶酸(tetrahydrofolate,THF)、5-甲基四氢叶酸(5-CH3-THF)、5-甲酰基四氢叶酸(5-CHO-THF)和5,10-亚甲基四氢叶酸(5,10-CH+THF)4种叶酸衍生物的含量。结果表明,4种叶酸衍生物在不同粒色的小麦中均有分布,总叶酸含量变异范围较广,变异系数为18.34%~21.10%,其中临4179总含量为66.99µg·100g^(-1),为高叶酸含量品种。5-CH3-THF和5-CHO-THF含量约为总叶酸含量的70%,且与总叶酸含量显著相关;紫粒和蓝粒品种的总叶酸平均含量分别为62.31和62.97µg·100 g^(-1),显著高于白粒(41.90µg·100 g^(-1))和红粒品种(44.36µg·100 g^(-1));基因型效应是影响总叶酸含量的主要因素,环境因素的影响较小。研究结果为小麦叶酸生物强化品种的选育和栽培提供了有价值的信息。Folic is essential for sustaining cell activity,promoting cell growth,and participating in cell division and proliferation.The demand for colored wheat is increasing due to its richness in anthocyanin,iron,zinc,selenium and other useful minerals.To research the content of folate in colored wheat grains,this study employed a total of 42 wheat varieties(lines)with varying grain colors as materials to ascertain the contents of 4 folic acid derivatives in 3 grains under 3 growing conditions:tetrahydrofolate(THF),5-methyltetrahydrofolate(5-CH3-THF),5-formyltetrahydrofolate(5-CHO-THF)and 5,10-methylenetetrahydrofolate(5,10-CH+THF).The results indicated that the 4 folic acid derivatives were distributed across wheat varieties with varying grain colors,with a coefficient of variation ranging from 18.34%to 21.10%,among which Lin 4179 had high folic acid content,and with a total content of 66.99µg·100 g^(-1).The contents of 5-CH3-THF and 5-CHO-THF accounted for approximately 70%of the total folic acid content.The mean total folate level in purple and blue grains was 62.31 and 62.97µg·100 g^(-1),respectively,which was significantly higher than that in white(41.90µg·100 g^(-1))and red grains(44.36µg·100 g^(-1)).Genotype effect was the main factor affecting total folic acid content,environmental factors have less impact.The results of this study provided an important insight for the development and breeding of folate biofortified wheat varieties.

关 键 词：小麦 粒色 叶酸 衍生物 

分 类 号：S512[农业科学—作物学]