基于质谱的单细胞蛋白质组学分析  

Single Cell Proteomic Analysis by Mass Spectrometry

作　　者：范智瑞 方群[1,2] 杨奕 FAN Zhirui;FANG Qun;YANG Yi(Department of Chemistry,Zhejiang University,Hangzhou 310058,China;ZJU-Hangzhou Global Scientific and Technological Innovation Center,Hangzhou 311215,China)

机构地区：[1]浙江大学化学系,杭州310058 [2]浙江大学杭州国际科创中心,杭州311215

出　　处：《高等学校化学学报》2024年第11期61-76,共16页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:22304153,22234007,21827806)资助。

摘　　要：单细胞蛋白质组学分析能揭示细胞个体之间蛋白质的精细差异,在诸多重要领域具有重要的应用价值,已成为目前的研究热点;其难点在于单细胞内的蛋白质极其微量,需要解决样品处理过程中的损失问题、色谱质谱检测的灵敏度问题和低信号强度质谱数据的解析利用问题.本文从单细胞分选、样品处理、色谱质谱采集和数据分析等方面,综合评述了目前基于质谱的单细胞蛋白质组学分析方法的研究进展及其在生物医学领域的应用,并展望了其未来的发展前景.Single-cell proteomics allows revealing precisely the differences of proteins between individual cells,which has become a research hotspot showing indispensable application value in many important fields.Its difficulties lie in the fact that the proteins in a single cell are of extremely low abundance,which calls for ingenious solutions to the problems of sample loss during preparation,low sensitivity of chromatography-mass spectrometry detection,and insufficient analysis of spectral data with low signal intensities.This review summarizes the current research progress of mass spectrometry-based single-cell proteomic analysis,including single-cell sorting,sample preparation,chromatography-mass spectrometry acquisition,and data analysis,as well as its applications in biomedical fields.Its potential future development is also discussed.

关 键 词：质谱 蛋白质组学 单细胞 微流控 数据非依赖性采集 

分 类 号：O657[理学—分析化学] Q516[理学—化学]