宫颈癌患者血清miR-410、miR-875表达与HPV感染的相关性  

Correlation between serum miR-410,miR-875 expression with HPV infection in cervical cancer patients

作　　者：曲书平 刘春君 梁菊华 QU Shuping;LIU Chunjun;LIANG Juhua(Gynecology,Yantai Yeda Hospital,Yantai,Shandong 264006,China)

机构地区：[1]烟台业达医院,山东烟台264006

出　　处：《中国病原生物学杂志》2024年第12期1404-1407,共4页Journal of Pathogen Biology

摘　　要：目的 探究宫颈癌患者血清miR-410、miR-875表达与人乳头状瘤病毒(HPV)感染的相关性。方法 选取2019年12月至2023年12月来本院治疗的87例宫颈癌患者为宫颈癌组,同期纳入65例宫颈上皮内瘤变患者为癌前病变组,另选取同期87例体检健康女性为对照组。采用荧光定量PCR法测定血清中miR-410、miR-875的水平;采用Pearson法分析血清miR-410、miR-875水平与HPV感染的相关性;Logistic回归分析宫颈癌发生的影响因素。结果 与对照组相比,宫颈癌组血清miR-410明显降低(P<0.05),miR-875水平明显升高(P<0.05);与癌前病变组相比,宫颈癌组血清miR-410明显降低(P<0.05),miR-875水平明显升高(P<0.05);与1~100组相比,100~1000组、>1000组血清miR-410明显降低(P<0.05),miR-875水平明显升高(P<0.05);与100~1000组相比,>1 000组血清miR-410明显降低(P<0.05),miR-875水平明显升高(P<0.05);流产史、miR-875为宫颈癌发生的独立危险因素(P<0.05),miR-410为独立保护因素(P<0.05)。结论 宫颈癌患者血清宫颈癌患者血清miR-410水平下调,与HPV负荷量呈负相关,为宫颈癌的独立保护因素;miR-875水平上调,与HPV负荷量呈正相关,为宫颈癌的独立危险因素,二者均可作为宫颈癌诊断的生物标志物。Objective To investigate the correlation between serum miR-410,miR-875 expression with human papillomavirus(HPV)infection in cervical cancer patients.Methods From December 2019toDecember 2023,87 cervical cancer patients treated in our hospital were included as the cervical cancer group,65 cervical intraepithelial neoplasia patients were included as the precancerous lesion group,and 87 healthy women who underwent physical examination were selected as the control group.Fluorescence quantitative PCR method was applied to determine the levels of miR-410 and miR-875 in serum.Pearson method was applied to analyze the correlation between serum miR-410,miR-875 levels with HPV infection.Logistic regression was applied to analyze the influencing factors of cervical cancer.Results Compared with the control group,the serum miR-410 in the cervical cancer group was greatly lower(P<0.05),and the miR-875 level was greatly higher(P<0.05).Compared with the precancerous lesion group,the serum miR-410 in the cervical cancer group was greatly lower(P<0.05),and the miR-875 level was greatly higher(P<0.05).Compared with the 1-100 group,the serum miR-410 level in the 100-1000 group and>1000 group was greatly lower(P<0.05),while the miR-875 level was greatly higher(P<0.05).Compared with the 100-1000 group,the serum miR-410 in the>1000 group was greatly lower(P<0.05)and the miR-875 level was greatly higher(P<0.05).Abortion history and miR-875 were independent risk factors for cervical cancer(P<0.05),while miR-410 was an independent protective factor(P<0.05).Conclusion Serum miR-410 level in cervical cancer patients is down-regulated and negatively correlated with HPV load,which is an independent protective factor for cervical cancer.The level of miR-875 is upregulated and positively correlated with HPV load,which is an independent risk factor for cervical cancer.Both can serve as biomarkers for the diagnosis of cervical cancer.

关 键 词：宫颈癌 miR-410 miR-875 人乳头状瘤病毒感染 

分 类 号：R737.33[医药卫生—肿瘤]