具有催化熊果苷酰化反应能力的微生物全细胞培养条件的优化  

Optimization of microbial whole cell culture conditions with catalytic activity for arbutin acylation

作　　者：吴涛 李一豪 胡珀[2] 何婷婷 赵祥杰 杨荣玲 李晓滟 朱伟杰 WU Tao;LI Yihao;HU Po;HE Tingting;ZHAO Xiangjie;YANG Rongling;LI Xiaoyan;ZHU Weijie(College of Life Science and Food Engineering,Huaiyin Institute of Technology,Huai’an 223001,China;Huai’an Food and Drug Inspection Institute,Huai’an 223001,China)

机构地区：[1]淮阴工学院生命科学与食品工程学院,江苏淮安223001 [2]淮安市食品药品检验所,江苏淮安223001

出　　处：《食品与发酵工业》2024年第22期137-142,共6页Food and Fermentation Industries

基　　金：国家自然科学基金项目(21706088);江苏省市场监督管理局科技计划项目(KJ2022048);江苏省大学生创新训练项目(202311049002Z)。

摘　　要：熊果苷是一种具有抗炎、抑菌、镇咳、美白等多种药理活性的糖苷类化合物,但其脂溶性差、生物利用度低,限制了其应用范围。全细胞催化其酰化反应,可获得具有生物活性好及生物利用度高的熊果苷酯衍生物。由于目前全细胞原始培养基配方所得的细胞生物量较低,故该研究以熊果苷辛酰化为反应模型,以米曲霉为菌种,探究了全细胞培养条件对细胞生物量及其催化效率的影响,以期获得较多生物量、较高催化活性的全细胞催化剂。结果表明,米曲霉在6.0 g/L大豆油、7.0 g/L胰蛋白胨、5.0 g/L(NH_(4))_(2)SO_(4)和0.2 g/L CaCl2的培养基中,于30℃、180 r/min培养48 h,米曲霉全细胞生物量达到6.28 g/L,较初始培养基提高了4.39倍,熊果苷转化率达99.55%。该研究设计的可催化熊果苷酰化反应的新型微生物全细胞培养体系,具有成本低、高效、高选择性等优点,为熊果苷酯衍生物的工业化制备提供了有效途径。Arbutin is a glycoside compound with various pharmacological activities such as anti-inflammatory,antibacterial,antitussive,and whitening,but its poor lipid solubility and low bioavailability limit its application range.The arbutin derivatives obtained by whole-cell catalysis have better bioactivity and bioavailability.However,the cell biomass from the original medium formula was low.In this paper,the effects of whole-cell culture conditions on cell biomass and catalytic efficiency were investigated by using arbutin caproylation as the reaction model and Aspergillus oryzae as the strain to obtain whole-cell catalysts with more biomass and higher catalytic activity.The biomass reached 6.28 g/L in the medium containing 6.0 g/L soybean oil,7.0 g/L tryptone,5.0 g/L(NH_(4))_(2)SO_(4),and 0.2 g/L CaCl 2 at 180 r/min and 30℃for 48 h,which was 4.39-folds higher than that in the initial conditions,and the arbutin conversion reached 99.55%.The new microbial whole-cell culture system that can catalyze arbutin acylation reaction has advantages including low cost,high efficiency,and high selectivity.This study provides an effective strategy for the industrial preparation of arbutin ester derivatives.

关 键 词：熊果苷 全细胞催化 酰化反应 培养条件优化 生物量 

分 类 号：Q813.11[生物学—生物工程] TQ28[化学工程—有机化工] TQ426.97