基于代谢组学分析Lentilactobacillus kefiri IMAU10804酸胁迫前后的代谢物差异  

Metabolomics-based analysis of Lentilactobacillus kefiri IMAU10804 metabolite differences before and after acid stress

作　　者：王会莹 薄晓宇 袁保壹 包秋华[1] WANG Huiying;BO Xiaoyu;YUAN Baoyi;BAO Qiuhua(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Inner Mongolia Agricultural University,Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Agricultural University,Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学,乳品生物技术与工程教育部重点实验室,农业农村部奶制品加工重点实验室,内蒙古自治区乳品生物技术与工程重点实验室,内蒙古呼和浩特010018

出　　处：《食品与发酵工业》2024年第22期197-203,213,共8页Food and Fermentation Industries

基　　金：内蒙古自然科学基金项目(2023MS03046);内蒙古硕士研究生科研创新项目(S20231094Z)。

摘　　要：酸胁迫制约着乳酸菌在生产应用过程中的活力及其益生功能的发挥,研究某一菌株的潜在耐酸机制对其进一步应用至关重要。该研究首先测定不同低pH值对开菲尔慢生乳杆菌(Lentilactobacillus kefiri)IMAU10804生长的影响,然后利用LC-MS技术从组学水平上分析L.kefiri IMAU10804酸胁迫前后的代谢物差异。结果显示,随着pH值的降低,L.kefiri IMAU10804生长受到抑制。将L.kefiri IMAU10804在pH 3.0的条件下处理60 min,正离子和负离子模式下分别检测到796和615种代谢物;采用SIMCA 14.1软件结合Metaboanalyst 5.0进行多元统计分析,大量代谢产物主要富集在ABC转运蛋白、精氨酸和脯氨酸代谢、嘌呤代谢等代谢通路。根据P<0.05、变量投影重要性≥1和变化倍数≥1.5原则筛选出L-丙氨酸、天冬氨酸、海藻糖等24种主要差异代谢物。该研究表明L.kefiri IMAU10804在酸胁迫环境下生长代谢物显著发生变化,可为后续乳酸菌产品开发和应用提供数据支撑。Acid stress restricts the viability and probiotic functions of lactic acid bacteria during production applications.Therefore,studying the potential acid tolerance mechanisms of a specific strain is crucial for its further application.This study first examined the influence of decreasing acidity on the growth of Lentilactobacillus kefiri IMAU10804,a slow-growing milk rod bacterium.Subsequently,this study used liquid chromatography-mass spectrometry(LC-MS)to analyze the metabolomics differences in L.kefiri IMAU10804 before and after acid stress.Results showed that the growth of L.kefiri IMAU10804 was inhibited with decreasing pH.When L.kefiri IMAU10804 was treated under pH 3.0 for 60 min,796 and 615 metabolites were detected in positive and negative ion modes,respectively.Multivariate statistical analysis was performed using SIMCA 14.1 software combined with Metaboanalyst 5.0,revealing that a large number of metabolites were enriched in metabolic pathways,such as ABC transporter,arginine and proline metabolism,and purine metabolism.Based on the criteria of P<0.05,variable importance in projection score≥1,and fold change≥1.5,24 major differential metabolites were found,including L-proline,aspartic acid,and trehalose.This study demonstrated significant changes in the growth and metabolism of L.kefiri IMAU10804 under acid stress environment,providing data support for the subsequent development and application of lactic acid bacteria products.

关 键 词：迟缓乳杆菌属 L.kefiri IMAU10804 酸胁迫 代谢组学 耐酸机理 

分 类 号：TS201.3[轻工技术与工程—食品科学]