基于元素组学的黔北麻羊羊腿肉的产地溯源可行性研究  

Feasibility discrimination traceability of leg meat origin in Qianbei brown goat based on elementomic

作　　者：张孝红 袁付明 周绍均 况晓宇 方鑫碧 何艳梅 邓勇 张建永 ZHANG Xiaohong;YUAN Fuming;ZHOU Shaojun;KUANG Xiaoyu;FANG Xinbi;HE Yanmei;DENG Yong;ZHANG Jianyong(College of Pharmacy,Zunyi Medical University,Zunyi 563000,China;Zunyi product quality inspection and Testing Institute,Zunyi 563000,China)

机构地区：[1]遵义医科大学药学院,贵州遵义563000 [2]遵义市产品质量检验检测院,贵州遵义563000

出　　处：《食品与发酵工业》2024年第22期204-213,共10页Food and Fermentation Industries

基　　金：贵州省科技厅项目(黔科合支撑[2022]一般090)。

摘　　要：基于元素组学分析黔北麻羊羊腿肉中的元素组成及含量,并与我国四大羊肉主产区对比分析,结合化学计量法进行产地溯源可行性研究。收集我国贵州、内蒙古、宁夏、新疆和甘肃5个产区的羊前腿肉共65份,采用微波消解法预处理后,通过电感耦合等离子体质谱法和电感耦合等离子体发射光谱法检测羊腿肉中17种元素的含量,结合单因素方差分析、主成分分析和正交偏最小二乘法判别分析对黔北麻羊进行产地区分,基于差异元素建立黔北麻羊产地判别模型。黔北麻羊与其余4个产区之间存在8种差异元素Mg、Al、K、Fe、P、Rb、Cr、Sn,建立了Fisher判别模型,对黔北麻羊的判别正确率为95%,采用“留一法”交叉验证对黔北麻羊的判别正确率为95%。通过8种差异元素建立的判别模型对黔北麻羊进行有效区分,元素组学可为黔北麻羊溯源分析提供新的参考。This study aimed to conduct a feasibility analysis on the traceability in the leg meat of Qianbei brown goat origin using elementomic,and compared with four major mutton-producing regions in China based on multi-element determined through stoichiometric methods.In total 65 foreleg cases of lamb were collected from Guizhou,Inner Mongolia,Ningxia,Xinjiang,and Gansu.The contents of 17 mineral elements were determined by inductively coupled plasma mass spectrometry(ICP-MS)and inductively coupled plasma-optical emission spectrometer(ICP-OES)after the mutton samples were pretreated by microwave digestion procedure.Combined with analysis of variance(ANOVA),principal component analysis(PCA),and orthogonal partial least squares discriminant(OPLS-DA),the Qianbei brown goat origins were distinguished.The discriminant model of mutton origin was established based on different elements.8 kinds of elements(Mg,Al,K,Fe,P,Rb,Cr,and Sn)in mutton were selected had significant differences between Guizhou Qianbei brown goat and four main mutton producing areas in China with OPLS-DA model.The Fisher discriminant function was established,and the cross-discrimination accuracy of samples in areas was 95.0%.The results showed that the 8 elements were feasible for the identification of the region Qianbei brown goat,thus providing a reference for traceability analysis.

关 键 词：羊肉 元素组学 黔北麻羊 产地溯源 化学计量法 

分 类 号：TS251.53[轻工技术与工程—农产品加工及贮藏工程] O657[轻工技术与工程—食品科学与工程]