栗瘿蜂体内一种合成生长素细菌的全基因组测序  

Whole Genome Sequencing of an Auxin-synthesizing Bacterium in Drycosmusk kuriphilus

作　　者：谢佳宜 杨雪梅 惠羽 朱道弘[2] 曾杨[2] 杨筱慧[1] XIE Jiayi;YANG Xuemei;HUI Yu;ZHU Daohong;ZENG Yang;YANG Xiaohui(College of Life Sciences,Hunan Normal University,Changsha 410081,Hunan,China;College of Life and Environmental Sciences,Central South University of Forestry and Technology,Changsha 410004,Hunan,China)

机构地区：[1]湖南师范大学生命科学学院,中国湖南长沙410081 [2]中南林业科技大学生命与环境科学学院,中国湖南长沙410004

出　　处：《生命科学研究》2024年第5期437-443,共7页Life Science Research

基　　金：国家重点研发计划项目(2018YFE0127100)。

摘　　要：致瘿昆虫体内高含量植物激素的来源尚不清楚。为探究细菌对栗瘿蜂(Drycosmusk kuriphilus)体内生长素合成的潜在影响,通过平板培养法分离出栗瘿蜂体内的两株细菌,即芽孢杆菌DK01(Bacillus sp.)和鞘氨醇杆菌DK02(Sphingobacterium sp.);使用第二代测序技术(Illumina HiSeq)与第三代单分子测序技术(PacBio)测定和组装了芽孢杆菌DK01的全基因组序列,并分析了芽孢杆菌DK01的生长素合成相关基因。研究结果表明,芽孢杆菌DK01基因组的全长为4244232 bp,GC含量为64.53%;该基因组存在3773个蛋白质编码区,编码了与生长素合成相关的乙醛脱氢酶、酰胺酶和腈水解酶。The source of the high levels of plant hormones in gall-inducing insects remains unknown at present.In order to explore the potential effect of bacteria on auxin synthesis in Drycosmusk kuriphilus,two strains of bacteria in D.kuriphilus,namely Bacillus sp.and Sphingobacterium sp.respectively,were isolated by the agar plate culture method.The whole genome sequence of Bacillus strain DK01 was determined and assembled using the second-generation sequencing(Illumina HiSeq)and third-generation single-molecule sequencing(PacBio),and the genes related to auxin synthesis were analyzed.The results showed that the total length of the Bacillus DK01 genome was 4244232 bp with a 64.53%GC content.There were 3773 protein-coding regions in the genome,and the auxin synthesis-related enzymes including aldehyde dehydrogenase,amidase,and nitrile hydrolase proved to be encoded.

关 键 词：栗瘿蜂 生长素 高效液相色谱-质谱技术(HPLC-MS) 全基因组测序 

分 类 号：Q968[生物学—昆虫学] S436.64[农业科学—农业昆虫与害虫防治]