Fusarium graminearum effector FgEC1 targets wheat TaGF14b protein to suppress TaRBOHD-mediated ROS production and promote infection  

作　　者：Shengping Shang Yuhan He Qianyong Hu Ying Fang Shifeng Cheng Cui-Jun Zhang 

机构地区：[1]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China [2]College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China

出　　处：《Journal of Integrative Plant Biology》2024年第10期2288-2303,共16页植物学报（英文版）

基　　金：supported by grants from the Elite Young Scientists Program of the CAAS (110243160001008);the Program for Guangdong “Zhu Jiang” Introducing Innovative and Entrepreneurial Teams (2019ZT08N628);the Shenzhen Science and Technology Program (AGIS-ZDKY202002)。

摘　　要：Fusarium head blight(FHB), caused by Fusarium graminearum, is a devastating disease of wheat globally. However, the molecular mechanisms underlying the interactions between F. graminearum and wheat remain unclear. Here, we identified a secreted effector protein, FgEC1, that is induced during wheat infection and is required for F. graminearum virulence. FgEC1 suppressed flg22-and chitin-induced callose deposition and reactive oxygen species(ROS) burst in Nicotiana benthamiana. FgEC1 directly interacts with TaGF14b, which is upregulated in wheat heads during F. graminearum infection. Overexpression of TaGF14b increases FHB resistance in wheat without compromising yield. TaGF14b interacts with NADPH oxidase respiratory burst oxidase homolog D(TaRBOHD) and protects it against degradation by the 26S proteasome. FgEC1 inhibited the interaction of TaGF14b with TaRBOHD and promoted TaRBOHD degradation, thereby reducing TaRBOHD-mediated ROS production.Our findings reveal a novel pathogenic mechanism in which a fungal pathogen acts via an effector to reduce TaRBOHD-mediated ROS production.

关 键 词：EFFECTOR FHB Fusarium graminearum reactive oxygen species WHEAT 

分 类 号：S435.121.45[农业科学—农业昆虫与害虫防治]