Revolutionizing stem cell research:unbiased insights through single-cell sequencing  

作　　者：HAO WU NA HUO SITUO WANG ZIWEI LIU YI JIANG QUAN SHI 

机构地区：[1]Institute of Stomatology,First Medical Center,Chinese PLA General Hospital,Beijing,100853,China

出　　处：《BIOCELL》2024年第11期1531-1542,共12页生物细胞（英文）

摘　　要：Stem cells have shown great application potential in wound repair,tissue regeneration,and disease treatment.Therefore,a full understanding of stem cells and their related regulatory mechanisms in disease treatment is conducive to improving the therapeutic effect of stem cells.However,thus far,there are still many unsolved mysteries in thefield of stem cells due to technical limitations,which hinder the in-depth exploration of stem cells and their wide clinical application.Single-cell sequencing(SCS)has provided very powerful and unbiased insights into cell gene expression profiles at the single-cell level,bringing exciting results to the stem cellfield.At present,SCS has been widely applied in thefield of stem cells,covering various aspects,including lineage tracing the development of stem cells,identifying new stem cell types,exploring cellular heterogeneity,and identifying internal functional subpopulations.In this paper,we focus on the latest research progress and discuss the application of SCS technology in stem cells.

关 键 词：Stem cell Single-cell sequencing Cellular heterogeneity SUBPOPULATIONS Functional analysis Lineage-tracing 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]