长臂猿感染H9N2亚型禽流感病毒病例报告  

A Case Report of Gibbon Infection with H9N2 Avian Influenza Virus

作　　者：冯华娟[1] 吴俊仪[1] 曹佳媛 尤宗耀[1] 杨露[1] 阙腾程 黄宁 廖小英 农汝[1] 黄泽琳[1] 陆兵兵[1] 李宁莉 莫亚生 FENG Huajuan;WU Junyi;CAO Jiayuan;YOU Zongyao;YANG Lu;QUE Tengcheng;HUANG Ning;LIAO Xiaoying;NONG Ru;HUANG Zelin;LU Bingbing;LI Ningli;MO Yasheng(Nanning Zoo,Nanning,530003,China;Faculty of Data Science City University of Macao,Macao,999078,China;Youjiang Medical University for Nationalities,Baise,533000,China;Guangxi Zhuang Autonomous Terrestrial Wildlife Rescue Research and Epidemic Diseases Monitoring Center,Nanning,530009,China;Liuzhou Zoo,Liuzhou,545005,China;Wuzhou Zoo,Wuzhou,543002,China)

机构地区：[1]南宁市动物园,南宁530003 [2]澳门城市大学数据科学学院,中国澳门999078 [3]右江民族医学院,百色533000 [4]广西壮族自治区陆生野生动物救护研究与疫源疫病监测中心,南宁530009 [5]柳州市动物园管理处,柳州545005 [6]梧州市动物园,梧州543002

出　　处：《野生动物学报》2024年第4期873-880,共8页CHINESE JOURNAL OF WILDLIFE

基　　金：广西野生动物救护与疫源疫病监测专题研究项目(GXZTYJXM202101)。

摘　　要：2022年1月,某动物园同一馆舍内饲养的6只长臂猿(Nomascus spp.)先后出现流清涕、咳嗽的症状。采集患病长臂猿鼻拭子共6份,利用高通量测序法对样本进行病原筛查,结果显示:2份样本呈AIV阳性,序列组装结果与H9N2亚型禽流感病毒的相似性最高;采用qPCR法对2份阳性样本进行H1~H16基因和N1~N9基因检测验证,发现5号样本H9 Ct值为31.35、N2 Ct值为36.16,6号样本H9 Ct值为27.46、N2 Ct值为29.57,均为阳性。给予患病长臂猿化痰止咳、消炎和控制继发感染等综合治疗,同时加强饲养环境消毒,患病长臂猿很快康复。In January 2022,six gibbons(Nomascus spp.)in the same enclosure of a zoo successively showed symptoms of runny nose and cough.We separately collected six nasal swabs from the six gibbons,detected the pathogens in the samples by high-throughput sequencing technology.The results showed that two samples were positive for AIV.The se⁃quence assembly was similar to H9N2 avian influenza virus.The two positive samples were tested by qPCR for H1-H16 genes and N1-N9 genes and the results showed that Ct values of H9 and N2 in sample 5 were 31.35 and 36.16 respec⁃tively,Ct values of H9 and N2 in sample 6 were 27.46 and 29.57.The gibbons were given comprehensive treatment,such as expectoration and cough relief,anti-inflammatory and secondary infection control,and disinfection of the feeding environ⁃ment was strengthened.The sick gibbons recovered quickly.

关 键 词：长臂猿 H9N2 禽流感病毒 高通量测序 qPCR 

分 类 号：S858.9[农业科学—临床兽医学]