香荚兰香草醛的理化分析和药物动力学  

Physicochemical Analysis and Pharmacokinetics of Vanillin from Vanilla planifolia

作　　者：陈梦蝶 吴亚楠 郭明[1] 程洁 CHEN Meng-Die;WU Ya-Nan;GUO Ming;CHENG Jie(College of Chemical and Materials Engineering,Zhejiang Agriculture and Forestry University,Hangzhou 311300,China)

机构地区：[1]浙江农林大学化学与材料工程学院,杭州311300

出　　处：《中国生物化学与分子生物学报》2024年第11期1596-1607,共12页Chinese Journal of Biochemistry and Molecular Biology

基　　金：浙江省“三农九方”科技协作计划项目资助(No.2024SNJF038)资助。

摘　　要：香荚兰的药用价值备受关注。本文分析香荚兰不同部位VOCs的共有主成分,以人血清白蛋白(human serum albumin,HSA)、β-乳球蛋白(β-lactoglobulin,β-La)及α-乳清蛋白(α-lactalbumin,α-La)为模板蛋白质,建立“固相微萃取气相色谱-质谱联用技术-多光谱技术-物理建模-药代动力学”(solid phase microextraction gas chromategraphy-mass spectrometry-multisp-ectral technique-physical modeling-pharmacokinetics,S-M-P-P)链式方法,解析香荚兰共有主成分的输运机制及药效机理。结果表明,香荚兰不同部位共有主VOCs为香草醛(vanillin,Van),其通过静态猝灭作用减弱了HSA/β-La/α-La的内源性荧光,并与HSA形成氢键和范德华力,与β-La/α-La则通过疏水作用力形成非共价复合物。两者间相互作用促进了Van在体内向肠道和肝组织的转运,并被CYP1A2和CYP2C9酶代谢,从而发挥其药理效果。该研究全面而深入地探讨了香荚兰VOCs的输运机制及药理效果,为理解植物源挥发性有机物的药用潜力提供了重要参考。The medicinal value of vanilla planifolia is of great interest.We analyzed the common principal components of VOCs in different parts of vanilla planifolia,and the human serum albumin(HSA),β-lactoglobulin(β-La)andα-lactalbumin(α-La)were used as template proteins to establish a chain analysis approach with the‘solid phase microextraction gas chromatography-mass spectrometry-multispectroscopy-physical modelling-pharmacokinetics’(S-M-P-P).The mechanisms of the transport and pharmacodynamics for the common principal components of vanilla planifolia were analyzed.The results showed that the common primary VOCs in different parts of vanilla planifolia was vanillin(Van),which attenuated the endogenous fluorescence of HSA/β-La/α-La by static bursting,and formed hydrogen bonds and Van der Waals forces with HSA,and noncovalent complexes withβ-La/α-La through hydrophobic forces.Their interaction facilitates the transport of Van in vivo to intestinal and hepatic tissues and its metabolism by CYP1A2 and CYP2C9 enzymes to exert its pharmacological effects.This study provides a comprehensive and in-depth investigation of the transport mechanisms and pharmacological effects of VOCs from vanilla planifolia,which provides an important reference for understanding the medicinal potential of plant derived VOCs.

关 键 词：香荚兰 多光谱技术 固相微萃取气相色谱-质谱联用技术 香草醛 

分 类 号：Q74[生物学—分子生物学]