贺兰山主要建群树种根际土壤真菌群落结构分析  

Analysis of Fungal Community Structure in Rhizosphere Soil of the Main Constructive Tree Species in Helan Mountain

作　　者：杨勋爵 孟兆云 李越 杨雨泽 李营刚 苏云 李敏[1,2] YANG Xunjue;MENG Zhaoyun;LI Yue;YANG Yuze;LI Yinggang;SU Yun;LI Min(College of Life Science and Technology,Inner Mongolia Normal University,Huhhot 010022,China;Key Laboratory of Biodi-versity Conservation and Sustainable Utilization in Mongolian Plateau for College and University of Inner Mongolia Autonomous Re-gion,Hohhot 010022,China;College of Geographical Science,Inner Mongolia Normal University,Huhhot 010022,China;Helan Mountain National Nature Reserve of Inner Mongolia,Bayanhot 750306,China)

机构地区：[1]内蒙古师范大学生命科学与技术学院,呼和浩特010022 [2]内蒙古自治区高等学校蒙古高原生物多样性保护与可持续利用重点实验室,呼和浩特010022 [3]内蒙古师范大学地理科学学院,呼和浩特010022 [4]内蒙古贺兰山国家级自然保护区管理局,内蒙古巴彦浩特750306

出　　处：《森林工程》2024年第6期20-29,共10页Forest Engineering

基　　金：内蒙古师范大学基本科研业务费专项资金资助(2022JBTD010);内蒙古师范大学研究生科研创新基金资助项目(CXJJS23058)。

摘　　要：贺兰山位于我国西北干旱半干旱地区,系统分析该地区土壤真菌群落的组成特点,对于明确干旱半干旱地区森林生态系统中真菌多样性及其群落功能具有重要意义。采用Illumina Miseq高通量测序技术,分析贺兰山3种主要建群树种青海云杉(Picea crassifolia)、油松(Pinus tabuliformis)和白桦(Betula platyphylla)的根际土壤真菌多样性和群落组成及其对土壤理化性质变化的响应。结果表明,从青海云杉、油松和白桦的根际土壤中分别获得真菌操作分类单位(operational taxonomic units,OTUs)数为267、353、433个,其中3个树种的共有OTUs数为60个。所有真菌OTUs属于17个门、42个纲、107个目、210个科、326个属。在门水平上,担子菌门(Basidiomycota)和子囊菌门(Ascomycota)真菌为主要优势真菌,相对丰度分别为58.7%和35.7%。在属水平上,相对丰度最高的5个属为蜡壳耳属(Sebacina,6.7%)、棉革菌属(Tomentella,6.5%)、丝盖伞属(Inocybe,6.1%)、双子囊菌属(Geminibasidium,3.7%)和青霉属(Penicillium,2.9%)。不同树种的优势真菌群落组成有显著差异,青海云杉、油松和白桦根际土壤中相对丰度最高的属分别为双子囊菌属(10.8%)、丝盖伞属(16.9%)和蜡壳耳属(9.0%)。采用冗余分析(redundancy analysis,RDA)分析贺兰山主要建群树种根际土壤真菌群落组成差异的驱动因素,其中土壤全氮含量和土壤含水量对真菌群落组成均有显著影响,解释度分别为41.6%和39.1%。贺兰山主要建群树种的土壤真菌多样性较高,与环境因子关系密切。研究结果丰富了贺兰山主要建群树种根际土壤真菌资源的生物信息,可以为干旱半干旱地区森林生态系统中真菌资源的挖掘和研究提供理论依据。Helan Mountain is located in the arid and semi-arid region of northwest China.Systematic analysis of the composition characteristics of soil fungal communities in this area is of great significance for clarifying the fungal diversity and community functions in forest ecosystems of arid and semi-arid regions.The Illumina Miseq high-throughput sequencing technology was used to analyze the fungal diversity,community composition,and their responses to changes of soil physicochemical properties in three main constructive tree species—Picea crassifolia,Pinus tabuliformis and Betula platyphylla in Helan Mountain.The results revealed that a total of 276,353 and 433 fungal OTUs were obtained from rhizosphere soil with 60 shared OTUs.All fungal OTUs belonged to 17 phyla,42 classes,107 orders,210 families and 326 genera.At the phylum level,Basidiomycota and Ascomycota were dominant,with relative abundances of 58.7%and 35.7%,respectively.At the genus level,the top five dominant genera were Sebacina(6.7%),Tomentela(6.5%),Inocybe(6.1%),Geminibasidium(3.7%)and Penicillium(2.9%).Significant differences were observed in the composi⁃tion of dominant fungi community at the genus level among different tree species.The genera with the highest relative abundance in the rhizosphere soil are Geminibasidium(10.8%),Inocybe(16.9%),and Sebacina(9.0%)respectively.RDA was used to analysis the driving factors of fungal community composition difference in the rhizosphere soil of the main constructive tree species in Helan Mountain.Soil total nitrogen content(TN)and soil water content(SWC)exert significant influences on the composition of fungal com⁃munities,accounting for explanation of 41.6%and 39.1%,respectively.This study enriches the biological information of fungal re⁃sources in the rhizosphere soil of the main tree species in Helan Mountain,and can provide theoretical basis for the excavation and re⁃search of fungal resources in forest ecosystems in arid and semi-arid areas.

关 键 词：建群树种 真菌多样性 群落结构 土壤理化性质 Illumina MiSeq测序 

分 类 号：S714.5[农业科学—林学]