基于均匀试验设计的小浆果多酚体外协同抗氧化活性研究  

Study on the Synergistic Antioxidant Activity of Small Berry Polyphenols in vitro Based on Uniform Design

作　　者：周丽萍[1] 于晓颖 何丹娆[1] 金欢欢 王化[1] ZHOU Liping;YU Xiaoying;HE Danrao;JIN Huanhuan;WANG Hua(Institute of Natural Resources and Ecology,Heilongjiang Academy of Sciences,Harbin 150040,China;School of Life Science,Heilongjiang University,Harbin 150080,China)

机构地区：[1]黑龙江省科学院自然与生态研究所,哈尔滨150040 [2]黑龙江大学生命科学学院,哈尔滨150080

出　　处：《森林工程》2024年第6期101-107,共7页Forest Engineering

基　　金：“十四五”国家重点研发计划(2022YFD1600502-16);黑龙江省省属科研院所业务费专项(ZNBZ2022ZZR09);黑龙江省科学院研究基金项目(KY2023ZR05)。

摘　　要：为研究4种小浆果多酚的协同抗氧化活性,选用均匀试验设计法优化小浆果多酚协同抗氧化组合,经过统计分析和数学回归模型拟合得到最优组合——笃斯越橘多酚(D2)、红豆越橘多酚(H2)、蓝靛果多酚(L2)、蔓越莓多酚(M2)最佳质量浓度分别为42、138、172、0μg/mL,对DPPH自由基的实际清除率为98.07%±0.31%,接近理论期望值100%,说明模型优选的组合具有可行性。通过协同效应分析,优选组合清除DPPH自由基和羟自由基的协同效应指数(Combination Index,CI)值分别为0.57和0.64,进一步说明笃斯越橘多酚(D2)、红豆越橘多酚(H2)、蓝靛果多酚(L2)3种浆果多酚具有显著协同抗氧化效果。研究成果可为天然抗氧剂及小浆果功能产品研发提供依据。To study the synergistic antioxidant activity of four small berry polyphenols,including Vaccinium uliginosum L.,Vac⁃ciniumvitis-idaea L.,Lonicera caerulea L.var.edulis Turcz.ex Herd.,and Vaccinium macrocarpon Ait.(labeled as D2,H2,L2,and M2,respectively),was studied.The uniform experimental design method was used to optimize the synergistic antioxidant combi⁃nation of small berry polyphenols.After statistical analysis and mathematical regression model fitting,the optimal combination was ob⁃tained as follows:the optimal concentrations of Vaccinium uliginosum L.polyphenol(D2),Vacciniumvitis-idaea L.polyphenol(H2),Lonicera caerulea L.var.edulis Turcz.ex Herd.Polyphenol(L2),and Vaccinium macrocarpon Ait.Polyphenol(M2)were 42μg/mL,138μg/mL,172μg/mL,and 0μg/mL,respectively.The actual clearance rate of DPPH free radicals was 98.07±0.31%,which was close to the theoretical expected value of 100%,indicating the feasibility of the selected combination in the model.Through synergistic effect analysis,the combination index(CI)values of the synergistic effect index of the selected combination for clearing DPPH and hydroxyl radicals were 0.57 and 0.64,indicated that the D2,H2,and L2 had significant synergistic antioxidant effects.The research results can provide a basis for the development of natural antioxidants and small berry functional products.

关 键 词：小浆果 多酚 均匀试验 协同效应分析 抗氧化 

分 类 号：S184[农业科学—农业基础科学]