整合素亚基-α3在头颈部鳞状细胞癌中的表达及临床意义  

Expression and clinical significance of ITGA3 in head and neck squamous cell carcinoma

作　　者：董惠萍 郝丽 翟兴华 阴旭斌 DONG Hui-ping;HAO Li;ZHAI Xing-hua;YIN Xu-bin(Nursing and Preschool Education Department of Shou-gang Technician College,Beijing 100144,China)

机构地区：[1]首钢技师学院护理与学前教育学院,北京100144 [2]首都医科大学附属北京天坛医院口腔科,100070

出　　处：《中华老年口腔医学杂志》2024年第5期262-268,共7页Chinese Journal of Geriatric Dentistry

摘　　要：目的通过在线数据库分析头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)中整合素亚基-α3(integrin subunit alpha 3,ITGA3)的表达水平及临床意义。方法利用TCGA和CPTAC肿瘤基因数据库分析HNSCC中ITGA3基因、蛋白和启动子甲基化水平与年龄、性别、种族、吸烟、临床分期与分级、淋巴结浸润、人乳头瘤病毒(human papilloma virus,HPV)感染及TP53突变的关系,利用UALCAN网站分析对HNSCC患者组织中的ITGA3的表达与总生存期的关系。结果与正常对照组织比较,在HNSCC患者标本中ITGA3表达升高,ITGA3启动子甲基化水平降低(P<0.001)。ITGA3基因和蛋白水平分别与患者性别、HPV感染、TP53突变、肿瘤分级、HIPPO通路变化、吸烟等相关。ITGA3表达水平升高与HNSCC患者总生存期缩短有关(P=0.0023);与相同ITGA3水平男性患者比较,女性患者总生存期呈缩短趋势(P=0.0021);随着肿瘤分级的升高,HNSCC患者总生存期呈缩短趋势(P=0.0013)。String数据库显示,ITGA3与10个基因相互作用,主要涉及细胞黏附和半桥粒组装。TIMER数据库显示,ITGA3水平与特定免疫细胞呈正相关(P<0.01)。结论ITGA3在HNSCC中是致瘤因素,表达量与性别、肿瘤分级、免疫细胞浸润、吸烟因素有关,有望成为治疗和预后的新靶点。Objective This study aims to analyze the expression levels and clinical significance of Integrin Subunit Alpha 3(ITGA3)in Head and Neck Squamous Cell Carcinoma(HNSCC)using online databases.Methods TCGA and CPTAC oncology gene databases were used to analyze the relationship between ITGA3 gene,protein,and promoter methylation levels in HNSCC and various tumor characteristics such as age,gender,race,smoking status,clinical stage,lymph node infiltration,HPV infection,and TP53 mutations.Additionally,the relationship between ITGA3 expression in HNSCC patient tissues and overall survival was analyzed using the UALCAN website.Results Compared with normal controls,ITGA3 expression was increased and ITGA3 promoter methylation was decreased in HNSCC patients(P<0.001).The expression levels of ITGA3 gene and protein were correlated with patient gender,HPV infection,TP53 mutations,tumor grading,HIPPO pathway alterations,and smoking status.Higher ITGA3 expression levels and female patients showed poorer prognoses.The String database revealed interactions between ITGA3 and 10 genes,primarily involved in cell adhesion and hemidesmosome assembly.Moreover,the TIMER database displayed a positive correlation between ITGA3 levels and specific immune cells.Conclusion ITGA3 functions as an oncogenic factor in HNSCC,with its expression associated with gender,tumor grading,immune cell infiltration,and smoking status.It holds potential as a novel therapeutic target and prognostic marker for HNSCC treatment and prognosis.

关 键 词：头颈部鳞状细胞 整合素亚基α3 总生存期 启动子甲基化 数据挖掘 

分 类 号：R782[医药卫生—口腔医学]