狼毒内生真菌Talaromyces sp.TP21活性次级代谢产物分析  

Bioactive Secondary Metabolites from Talaromyces sp.TP21,an Endophytic Fungus of Stellera chamaejasme

作　　者：王子墨 刘博 王晓晴 张丹丹 张璇 康雅男 孙奕[1] WANG Zimo;LIU Bo;WANG Xiaoqing;ZHANG Dandan;ZHANG Xuan;KANG Yanan;SUN Yi(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2024年第23期205-213,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院科技创新工程项目(CI2021A04514);国家自然科学基金项目(81502968)。

摘　　要：目的:对狼毒内生真菌Talaromyces sp.TP21的次级代谢产物及其生物活性进行研究。方法:采用正、反相柱色谱法和高相液相色谱法,结合分子网络(MN)分析与生物活性导向分离TP21的次级代谢产物,并综合运用核磁共振波谱(NMR)、高分辨质谱(HR MS)等光谱法确定化合物结构。采用噻唑蓝(MTT)比色法,测定所有化合物抑制肺癌细胞株A549和肝癌细胞株Hep G2生长的作用,同时评价其对金黄葡萄球菌和人口腔源酿酒酵母菌的抑菌活性。结果:从狼毒内生真菌Talaromyces sp.TP21次级代谢物中分离鉴定出17个化合物,分别为ergochrome C(1)、daldiniaeschsone A(2)、seco-blennolide B(3)、penitholabene(4)、penicichrysogene A(5)、orsellinic acid(6)、griseofulvin(7)、isorhodoptilometrin(8)、(+)-5-chloromitorubrinic acid(9)、penicillixanthone A(10)、pentacecilide B(11)、pentacecilide C(12)、chrodrimanin C(13)、chrodrimanin E(14)、chrodrimanin H(15)、chrodrimanin F(16)、3-hydroxypentacecilide A(17)。结论:化合物1~5,11~12,17均为首次从篮状菌属中分离得到。其中,化合物4、7、10~12具有抑制A549和Hep G2细胞生长作用[半抑制浓度(IC50)<50μmol·L^(-1)],化合物9、10对金黄葡萄球菌及人口腔源酿酒酵母菌具有抑菌活性[最小抑制浓度(MIC)8~128 mg·L^(-1)]。Objective:To study the bioactive secondary metabolites of Talaromyces sp.TP21 and their bioactivities.Method:The secondary metabolites of Talaromyces sp.TP21 were isolated by high performance liquid chromatography(HPLC),normal phase and reversed phase column chromatography combined with molecular networking and bioassay-guided fractionation,and their structures were determined by nuclear magnetic resonance(NMR)and high resolution mass spectrometry(HR MS).The inhibitory effects of the compounds on the growth of the lung cancer cell line A549 and the liver cancer cell line Hep G2 were measured by themethyl thiazolyl tetrazolium(MTT)method.The antimicrobial activities of the compounds were measured with Staphylococcus aureus and human oral cavity-derived Saccharomyces cerevisiae as the indicator microorganisms.Result:Seventeen compounds were isolated from the secondary metabolites of Talaromyces sp.TP21 and identified as ergochrome C(1),daldiniaeschsone A(2),seco-blennolide B(3),penitholabene(4),penicichrysogene A(5),orsellinic acid(6),griseofulvin(7),isorhodoptilometrin(8),(+)-5-chloromitorubrinic acid(9),penicillixanthone A(10),pentacecilide B(11),pentacecilide C(12),chrodrimanin C(13),chrodrimanin E(14),chrodrimanin H(15),chrodrimanin F(16),and 3-hydroxypentacecilide A(17).Conclusion:Compounds 1-5,11-12,and 17 were isolated from Talaromyces for the first time.Among them,compounds 4,7,and 10-12 exhibited inhibitory activities against the growth of A549 and Hep G2 cells,with the median inhibitory concentration below 50μmol·L^(-1).Furthermore,compounds 9 and 10 showed inhibitory activities against S.aureus and human oral cavity-derived S.cerevisiae,with the minimum inhibitory concentration of 8-128 mg·L^(-1).

关 键 词：狼毒 内生真菌 Talaromyces sp. 次级代谢产物 抗肿瘤活性 抑菌活性 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学] R289R287R22R2-031R33R24