lncRNA-miRNA-mRNA在肺腺癌合并肺栓塞中的作用  

The role of lncRNA-miRNA-mRNA in pulmonary adenocarcinoma complicated with pulmonary embolism

作　　者：何丽丽 张穗 牛海文 曹国磊[1] 蔡倩 罗琴[1] He Lili;Zhang Sui;Niu Haiwen;Cao Guolei;Cai Qian;Luo Qin(Department of Respiratory Neurology,Affiliated Tumor Hospital of Xinjiang Medical University,Urumqi83001l,China)

机构地区：[1]新疆医科大学附属肿瘤医院呼吸神经内科,乌鲁木齐830011

出　　处：《国际呼吸杂志》2024年第10期1164-1173,共10页International Journal of Respiration

基　　金：新疆维吾尔自治区自然科学基金(2022D01D74);新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目(WJWY-202421)

摘　　要：目的分析长链非编码RNA(lncRNA)-微RNA(miRNA)-mRNA在肺腺癌合并肺栓塞中的作用。方法本研究为横断面研究,采用非随机抽样的方法选取2019年1~12月于新疆医科大学附属肿瘤医院住院的肺腺癌患者(肺腺癌组,4例)和肺腺癌合并肺栓塞患者(肺栓塞组,4例),以及同期接受体检的健康对照者(健康对照组,4例)为研究对象。采集所有受试者的外周静脉血,提取外周血细胞中的总RNA,测定总RNA的纯度和浓度。构建RNA文库并进行全基因组测序,分析差异表达的lncRNA、miRNA和mRNA,并对3组间差异基因取交集。预测miRNA和lncRNA的靶基因,对靶基因进行KEGG通路分析和靶基因通路富集分析。构建lncRNA-miRNA共表达网络,预测内源竞争RNA(ceRNA)并构建lncRNA-miRNA-ceRNA网络。结果肺腺癌组男1例,女3例,年龄56.5(49.0,66.5)岁;肺栓塞组男2例,女2例,年龄46.5(39.5,55.0)岁;健康对照组男2例,女2例,年龄51.0(50.0,55.0)岁。与健康对照组相比,肺腺癌组观察到425个上调lncRNA、93个上调miRNA和1013个上调mRNA,同时观察到379个下调lncRNA、17个下调miRNA和1285个下调mRNA;与健康对照组相比,肺栓塞组观察到453个上调lncRNA、65个上调miRNA和1327个上调mRNA,同时观察到737个下调lncRNA、34个下调miRNA和1674个下调mRNA;与肺腺癌组相比,肺栓塞组观察到326个上调lncRNA、30个上调miRNA和1181个上调mRNA,同时观察到399个下调lncRNA、61个下调miRNA和871个下调mRNA。对上述差异基因取交集,结果显示85个lncRNA和33个miRNA在3组间均差异表达,其中2个miRNA和7个lncRNA被鉴定为可能靶向不同功能的特定基因。与健康对照组相比,肺腺癌组靶基因富集的通路包括基因表达、蛋白质代谢和信号通路等;与肺腺癌组相比,肺栓塞组靶基因富集的通路包括基因表达、代谢和蛋白质代谢等。MERGE.20388.1与4个miRNA、hsa-miR-511-5p与4个lncRNA之间存在调控关系,且在MERGE.20388.1、hsa-miR-511-5p中�ObjectiveTo analyze the role of long noncoding RNA(lncRNA)-microRNA(miRNA)-mRNA in lung adenocarcinoma complicated with pulmonary embolism(PE).MethodsThis was a cross-sectional study involving lung adenocarcinoma(lung adenocarcinoma group,4 cases),lung adenocarcinoma patients combined with PE(PE group,4 cases)hospitalized in the Affiliated Tumor Hospital of Xinjiang Medical University from January to December 2019,and healthy controls who received physical examination during the same period(healthy control group,4 cases)by non-random sampling.Peripheral venous blood was collected to extract total RNA,and the purity and concentration of total RNA were determined.An RNA library was constructed and genome-wide sequencing was performed to analyze differentially expressed lncRNAs,miRNAs and mRNAs,and the intersection of differentially expressed genes among the three groups was taken.Target genes of differentiability expressed miRNAs and lncRNAs were predicted and subjected to Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis.A lncRNA-miRNA co-expression network was depicted,and the competing endogenous RNAs(ceRNAs)were predicted to build a lncRNA-miRNA-ceRNA network.ResultsThere was 1 male and 3 females with a mean age of 56.5(49.0,66.5)years in the lung adenocarcinoma group;2 males and 2 females with a mean age of 46.5(39.5,55.0)years in the PE group;2 males and 2 females with a mean age of 51.0(50.0,55.0)years in the healthy control group.Compared with the healthy control group,425 up-regulated lncRNAs,93 up-regulated miRNAs and 1013 up-regulated mRNAs were observed in the lung adenocarcinoma group,while 379 down-regulated lncRNAs,17 down-regulated miRNAs and 1285 down-regulated mRNAs were observed.Compared with the healthy control group,453 up-regulated lncRNAs,65 up-regulated miRNAs and 1327 up-regulated mRNAs were observed in the PE group,while 737 down-regulated lncRNAs,34 down-regulated miRNAs and 1674 down-regulated mRNAs were observed.Compared with the lung adenocarcinoma group,326 up-regulated

关 键 词：肺腺癌 肺栓塞 RNA 未翻译 微RNAS 长链非编码RNA 

分 类 号：R734.2[医药卫生—肿瘤] R563.5[医药卫生—临床医学]