四种忍冬属植物叶绿体基因组结构特征及系统发育分析  

Structural characteristics and phylogenetic analysis of chloroplast genomes of four species of Lonicera

作　　者：熊瑶[1] 童凌斐[1] 曹岚 慕泽泾 沈成英 杜小浪 XIONG Yao;TONG Ling-fei;CAO Lan;MU Ze-jing;SHEN Cheng-ying;DU Xiao-lang(Department of Pharmacy,Jiangxi Provincial People's Hospital,the First Affiliated Hospital of Nanchang College,Nanchang 330006,China;Research Center of Chinese Herbal Medicine and Ethnic Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西省人民医院(南昌医学院第一附属医院)药学部,江西南昌330006 [2]江西中医药大学,中药资源与民族药研究中心,江西南昌330004

出　　处：《药学学报》2024年第11期3164-3171,共8页Acta Pharmaceutica Sinica

基　　金：国家科技基础资源调查专项(2018FY100703);江西中医院大学校级科技创新团队发展计划(CXTD22002)。

摘　　要：忍冬属(Lonicera)是忍冬科(Caprifoliaceae)最大的属,忍冬科药用植物在我国历史悠久,资源丰富。由于具有观赏和药用价值,忍冬的经济价值十分突出。然而,受巨大需求和高价刺激的影响,市场上出现了严重的混杂现象。本文通过高通量测序技术对越橘叶忍冬[L.angustifolia Wallich ex Candolle var.myrtillus(Hook.f.&Thomson)Q.E.Yang]、圆叶忍冬(L.myrtillusHook.f.et Thoms.var.cyclophyllaRehd)、四川忍冬(L.szechuanica Batal)和唐古特忍冬(L.tangutica Maxim)的叶绿体基因组进行测序和组装,并对其进行结构比较和系统发育学研究。结果显示,4种忍冬的叶绿体基因组呈典型的环状四分体结构,总长度为154608~163413 bp,总GC含量为37.93%~38.42%。共注释128~129个基因,包括83~84个蛋白编码基因、8个rRNA基因和37个tRNA基因。忍冬属叶绿体重复结构分析共检测到53~68个简单重复序列(simple sequence repeats,SSRs)和133~745个长重复序列。系统发育研究表明,21种忍冬属药用植物可以明显聚为一支,其中越橘叶忍冬和四川忍冬的亲缘关系较近,圆叶忍冬和唐古特忍冬的亲缘关系较近。本研究首次对忍冬属叶绿体基因组及系统发育关系进行了全面的研究,为揭示忍冬属物种间的遗传信息、物种进化和遗传多样性研究提供一定的科学依据。Lonicera Linn.is the largest genus of family Caprifoliaceae,which has a long history and abundant resources in China.Due to its ornamental and medicinal properties,the species of Lonicera shows outstanding economic value.However,affected by the huge demand and high price stimulation,there is a serious mixing phenomenon on the market.In this study,high-throughput sequencing technology was used to analyze the analysis of L.angustifolia Wallich ex Candolle var.myrtillus(Hook.f.&Thomson)Q.E.Yang,L.myrtillus Hook.f.et Thoms.var.cyclophylla Rehd,L.szechuanica Batal and L.tangutica Maxim to sequence and assemble their chloroplast(CP)genomes,and to conduct structural comparisons and phylogenetic studies.The results showed that the chloroplast genomes of the four species showed a typical circular tetrad structure,with a total length of 154608-163413 bp and a total GC content of 37.93%-38.42%.A total of 128-129 genes were annotated,including 83-84 protein-coding genes,8 rRNA genes,and 37 tRNA genes.A total of 53-68 SSRs and 133-745long repeats were detected by chloroplast repeat structure analysis.Phylogenetic studies showed that 21 species of Lonicera medicinal plants could be significantly clustered into one branch,among which the relatives of L.angustifolia and L.szechuanica were close,and the kinship of L.myrtillus and L.tangutica was close.This study is the first comprehensive study of the chloroplast genome and phylogenetic relationship of Loicera species,and the experimental results provide a scientific basis for revealing the genetic information,species evolution and genetic diversity of Lonicera species.

关 键 词：忍冬 叶绿体基因组 比较分析 系统发育关系 

分 类 号：R931[医药卫生—生药学]