槲皮素提高人结直肠癌细胞SW480和SW620对顺铂的敏感性  

Quercetin increases the sensitivity of human colon cancer cells SW480 and SW620 to cisplatin

作　　者：周锋 沙德胜 卢瑗瑗 陈维 ZHOU Feng;SHA Desheng;LU Yuanyuan;CHEN Wei(Department of General Surgery,The People’s Hospital of Rugao,Rugao 226500;Department of Gastroenterology,Xijing Hospital of Digestive Diseases,Air Force Medical University,Xi’an 710032;Department of Oncology,The People’s Hospital of Rugao,Rugao 226500,China)

机构地区：[1]南通大学附属如皋医院/如皋市人民医院普通外科,江苏如皋226500 [2]空军军医大学西京消化病医院消化内科,陕西西安710032 [3]南通大学附属如皋医院/如皋市人民医院肿瘤内科,江苏如皋226500

出　　处：《西安交通大学学报（医学版）》2024年第6期902-908,共7页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金面上项目(No.81871913);南通市卫生健康委员会面上课题B资助项目(No.MB2021085)。

摘　　要：目的本研究旨在探究槲皮素对顺铂耐药人结直肠癌(colorectal cancer,CRC)细胞SW480和SW620的抑制作用及其可能的机制。方法培养SW480和SW620细胞的顺铂耐药亚型,并用MTT法测定槲皮素对细胞增殖和化疗敏感性的影响。流式细胞术用于检测细胞凋亡,蛋白质印迹法用于检测相关蛋白质的表达。结果MTT实验显示,80μmol/L和160μmol/L浓度的槲皮素显著抑制了SW480和SW620细胞的增殖。流式细胞术结果表明,顺铂联合槲皮素组的细胞凋亡率明显高于其他3组。蛋白质印迹法结果显示,顺铂联合槲皮素组的裂解型半胱天冬酶-3(cleaved-caspase-3)和半胱天冬酶-9(caspase-9)表达显著增加。化疗药物敏感性检测结果表明,耐药细胞的IC_(50)升高,而在槲皮素给药后显著下降(P均<0.05)。结论本研究明确了槲皮素能够提高人CRC细胞对顺铂的敏感性。这种效应可能与其影响细胞增殖、凋亡和自噬等方面的作用机制有关。Objective To investigate the inhibitory effect of quercetin on cisplatin-resistant human colorectal cancer(CRC)cells SW480 and SW620 and its possible mechanism.Methods Cisplatin-resistant subtypes of SW480 and SW620 cells were first cultured and the effects of quercetin on cell proliferation and chemosensitivity were determined by MTT assay.Flow cytometry was used to detect apoptosis and protein blotting was used to detect the expressions of relevant proteins.Results MTT assay showed that quercetin at the concentrations of 80μmol/L and 160μmol/L significantly inhibited the proliferation of SW480 and SW620 cells.Flow cytometry results showed that the apoptosis rate was significantly higher in the cisplatin combined with quercetin group than in the other three groups.Protein blotting showed that cleaved-caspase-3 and caspase-9 expressions were significantly increased in the cisplatin combined with quercetin group.The chemotherapeutic drug sensitivity assay showed that the elevated IC_(50)of drug-resistant cells was decreased significantly after quercetin administration(P<0.05).Conclusion The present study clarifies that quercetin can increase the sensitivity of human CRC cells to cisplatin.This effect may be related to its mechanism of action in affecting cell proliferation,apoptosis and autophagy.

关 键 词：槲皮素 SW480细胞 SW620细胞 结直肠癌(CRC) 顺铂 

分 类 号：R735.35[医药卫生—肿瘤]