5种多糖对木糖葡萄球菌增益作用及体外共发酵特性作用机制  

Enhancement Effects and in vitro Co-fermentation Characteristics of Five Plant-Derived Polysaccharides on Staphylococcus xylosus

作　　者：王杰[1] 蔡奕贤 何建章[1] 陈良华[1] 张帮周 WANG Jie;CAI Yixian;HE Jianzhang;CHEN Lianghua;ZHANG Bangzhou(Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant,Fujian Institute of Subtropical Botany,Xiamen 361006,Fujian,China;Xiamen Treatgut Biotechnology Co.,Ltd.,Xiamen 361000,Fujian,China;Xiamen United Respiratory Health Institute,Xiamen 361000,Fujian,China)

机构地区：[1]福建省亚热带植物研究所福建省亚热带植物生理生化重点实验室,福建厦门361006 [2]厦门承葛生物科技有限公司,福建厦门361000 [3]厦门联合呼吸健康研究院,福建厦门361000

出　　处：《食品研究与开发》2024年第22期62-69,共8页Food Research and Development

基　　金：厦门市重大科技计划项目(3502Z20211005)。

摘　　要：探究蒲公英多糖、黄芪多糖、枸杞多糖、山药多糖与当归多糖5种多糖对木糖葡萄球菌(Staphylococcus xylosus)TG022增殖和产酶能力的影响,进一步探究蒲公英多糖、当归多糖与木糖葡萄球菌在肠道微生物调节中的作用。分别配制以上述5种多糖作为唯一碳源的培养基,与木糖葡萄球菌TG022共同培养,以菌株生长曲线、pH值、酶活为指标,筛选对木糖葡萄球菌TG022有增益作用的多糖;通过体外模拟肠发酵试验,探究筛选的多糖对肠道微生物的影响。结果表明,当归多糖和蒲公英多糖对木糖葡萄球菌TG022的协同增长效果最优,其生长曲线与酶活表现出协同增加的趋势,其他3种多糖对木糖葡萄球菌TG022无协同增长效果;体外模拟肠道发酵液的16S rRNA基因高通量测序结果表明,与阴性对照组相比,添加当归多糖与蒲公英多糖后发酵的木糖葡萄球菌显著降低了致病菌属的相对丰度,增加了有益菌属相对丰度。蒲公英多糖和当归多糖能够作为唯一的碳源促进木糖葡萄球菌TG022生长,且在菌株的增殖量、酶活、体外肠道模拟发酵中协同发挥较好作用。The proliferation and enzyme production of Staphylococcus xylosus TG022 treated with polysaccha-rides from five polysaccharides(Taraxaci Herba,Astragali Radix,Lycii Fructus,Dioscoreae Rhizoma,and An-gelicae Sinensis Radix)with both edible and medicinal values were studied.The regulating effects of Taraxaci Herba polysaccharide and Angelicae Sinensis Radix polysaccharide combined with S.xylosus TG022 on intesti-nal flora were explored.The five polysaccharides were respectively used as the sole carbon sources for the cul-ture of S.xylosus TG022,and the polysaccharides suitable for strain growth was screened out based on the growth curve,pH,and enzyme activity.Furthermore,in vitro intestinal fermentation experiments were carried out to examine the effects of the polysaccharides screened out on intestinal flora.The results showed that An-gelicae Sinensis Radix polysaccharide and Taraxaci Herba polysaccharide had the best effects on the growth of S.xylosus TG022,increasing the biomass and enzyme activity.The polysaccharides from the other three Chi-nese herbal medicines had no promoting effect on the growth of S.xylosus TG022.The results of 16S rRNA gene high-throughput sequencing showed that compared with the negative control,fermentation of S.xylosus TG022 with Angelicae Sinensis Radix or Taraxaci Herba polysaccharide reduced the relative abundance of pathogenic microorganisms and increased the relative abundance of beneficial microorganisms.Angelicae Si-nensis Radix or Taraxaci Herba polysaccharide could be used as the only carbon source to promote the growth of S.xylosus TG022,promoting the proliferation,enzyme production,and in vitro intestinal fermentation.S.xy-losus TG022 combined with each of the two polysaccharides can regulate the relative abundance of pathogenic microorganisms and beneficial microorganisms in the intestine.

关 键 词：药食同源 多糖 木糖葡萄糖球菌 肠道菌群 体外共发酵 抗氧化活性 

分 类 号：TS201.3[轻工技术与工程—食品科学]