扶芳藤提取物联合奥沙利铂对结肠癌IKKβ/NF-κB信号通路的影响  

The effect of Euonymus Fortunei extract combined with oxaliplatin on the IKKβ/NF-κB signaling pathway in colon cancer

作　　者：刘家旋 剧永乐 廖嘉宝 欧阳满照 罗振涛 廖天佑 黄华聪 Liu Jiaxuan;Ju Yongle;Liao Jiabao;Ouyang Manzhao;Luo Zhentao;Liao Tianyou;Huang Huacong(Department of Gastrointestinal Surgery,Shunde Hospital of Southern Medical University(The First People’s Hospital of Shunde),Foshan 528300,Guangdong,China)

机构地区：[1]南方医科大学顺德医院(佛山市顺德区第一人民医院)胃肠外科,广东佛山528300

出　　处：《结直肠肛门外科》2024年第5期607-611,共5页Journal of Colorectal & Anal Surgery

基　　金：2022年佛山市自筹经费类科技计划项目(2220001004063)。

摘　　要：目的探讨扶芳藤提取物联合奥沙利铂对结肠癌肿瘤生长及炎症因子表达的影响。方法选取BALB/c裸鼠皮下注射结肠癌CT26细胞建立结肠癌荷瘤模型。将32只裸鼠随机分为荷瘤模型组、奥沙利铂干预组、扶芳藤干预组、联合干预组,每组8只,连续干预3周,测量肿瘤体积、肿瘤质量并计算抑瘤率。采用ELISA法检测血清中IL-1、IL-6、TNF-α的水平,Western blot法检测肿瘤组织中IKKβ/NF-κB信号通路蛋白的表达。结果干预结束时,与荷瘤模型组相比,扶芳藤干预组的肿瘤体积和肿瘤质量无明显差异(P>0.05),但血清IL-1、IL-6、TNF-α水平和肿瘤组织中IKKβ、NF-κB相对表达量显著降低(P<0.05)。与奥沙利铂干预组相比,联合干预组的肿瘤体积和肿瘤质量显著降低(P<0.05),肿瘤抑制率显著增加(69.26%),血清IL-1、IL-6、TNF-α水平及IKKβ、NF-κB相对表达量显著降低(P<0.05)。结论扶芳藤单药治疗对结肠癌肿瘤生长无明显抑制,但其联合奥沙利铂对结肠癌肿瘤生长有显著抑制作用,这可能与扶芳藤提取物对肿瘤IKKβ/NF-κB信号通路的调控有关。Objectives To investigate the effects of Euonymus Fortunei extract combined with oxaliplatin on tumor growth and the expression of inflammatory cytokines in colon cancer.Methods BALB/c nude mice were subcutaneously injected with CT26 colon cancer cells to establish a xenograft model of colon cancer.Thirty-two nude mice were randomly divided into four groups:the tumor-bearing group,the oxaliplatin intervention group,the Euonymus Fortunei intervention group,and the combined intervention group(Euonymus Fortunei combined with oxaliplatin),with 8 mice in each group.The interventions were continuously administered for 3 weeks.Tumor volume,tumor mass were measured,and the tumor inhibition rate was calculated.The levels of IL-1,IL-6,and TNF-αin serum were measured using ELISA.The expression of IKKβand NF-κB proteins in tumor tissues was detected by Western blotting.Results Compared with the tumor-bearing group,the Euonymus Fortunei intervention group showed no significant difference in tumor volume and tumor mass(P>0.05),but the levels of IL-1,IL-6,and TNF-αin serum and the relative expression of IKKβand NF-κB proteins in tumor tissues were significantly reduced(P<0.05).Compared with the oxaliplatin intervention group,the combined intervention group showed significantly reduced tumor volume and tumor mass(P<0.05),and a significantly increased tumor inhibition rate(69.26%).The levels of IL-1,IL-6,and TNF-αin serum and the relative expression of IKKβand NF-κB proteins in tumor tissues were significantly reduced(P<0.05).Conclusion Although monotherapy with Euonymus Fortunei did not significantly inhibit tumor growth in colon cancer,its combination with oxaliplatin showed a significant inhibitory effect on tumor growth.This effect may be related to the regulation of the IKKβ/NF-κB signaling pathway by Euonymus Fortunei extract.

关 键 词：结肠癌 扶芳藤 奥沙利铂 IKKβ/NF-κB信号通路 

分 类 号：R735.3[医药卫生—肿瘤]