转录组与代谢组联合分析揭示大豆GmERD15c参与盐胁迫下类黄酮的生物合成  

Revealing the Flavonoid Biosynthesis of Soybean GmERD15c under Salt Stress by Combined Analysis of Transcriptome and Metabolome

作　　者：韩乐乐 宋文迪 边嘉珅 李阳 杨双胜 陈紫怡 李晓薇[1,2] HAN Le-le;SONG Wen-di;BIAN Jia-shen;LI Yang;YANG Shuang-sheng;CHEN Zi-yi;LI Xiao-wei(College of Life Sciences,Jilin Agricultural University,Changchun 130118;Technology Innovation Laboratory for Creation and High-value Utilization of Oil Germplasm Resources,Changchun 130118)

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]油料种质资源创制及高值化利用技术创新实验室,长春130118

出　　处：《生物技术通报》2024年第10期243-252,共10页Biotechnology Bulletin

基　　金：吉林省科技厅项目(20220402049GH);吉林省大学生创新训练项目(S202310193021)。

摘　　要：【目的】大豆GmERD15c是ERD15转录因子家族成员之一,探究盐胁迫下转GmERD15c基因大豆株系的基因表达及物质代谢情况,揭示其与大豆耐盐性的关系。【方法】以转GmERD15c基因大豆株系及其受体大豆‘东农50’为材料,经盐胁迫处理后,采取根部进行转录组学和代谢组学分析。【结果】盐胁迫前后差异代谢物在类黄酮合成通路变化较大,胁迫后的代谢产物二氢山奈酚、儿茶素和槲皮素含量发生显著变化,且槲皮素含量在转GmERD15c基因株系中更高。同时,转录组学与代谢组学联合分析发现,GmERD15c基因与类黄酮生物合成相关酶关系密切。【结论】盐胁迫下,大豆GmERD15c基因可能通过调控类黄酮合成相关的酶类进而影响类黄酮的生物合成,而类黄酮可以减轻盐分引起的氧化应激,为后续揭示GmERD15c基因的耐盐机制提供了新线索。【Objective】Soybean GmERD15c is one of the members of the ERD15 transcription factor family,and the gene expression and substance metabolism of soybean lines transgenic with GmERD15c gene under salt stress were explored,and the relationship between GmERD15c and soybean's tolerance to salt was revealed.【Method】Using the transgenic soybean line and its recipient soybean‘Dongnong 50'as the materials,and the roots after the salt stress treatment were to have analysis of transcriptomics and metabolomics.【Result】There were significant changes in the metabolites of flavonoid synthesis pathway before and after salt stress,with significant variations in the content of dihydroquercetin,catechin,and fisetin after stress.And the content of quercetin was higher in the transgenic line of GmERD15c.Meanwhile,the combined analysis of transcriptomics and metabolomics revealed that the GmERD15c gene was closely related to the flavonoid biosynthesisrelated enzymes.【Conclusion】It is hypothesized that the soybean GmERD15c gene may affect the biosynthesis of flavonoids by regulating the enzymes related to flavonoid synthesis under salt stress,and flavonoids may reduce the oxidative stress caused by salt,which provides new clues for subsequently revealing the salt tolerance mechanism of the GmERD15c gene.

关 键 词：大豆 GmERD15 类黄酮 转录组学 代谢组学 

分 类 号：S565.1[农业科学—作物学] Q943.2[生物学—植物学]