基于生物信息学分析TFF3在肺腺癌中的表达和调控作用  

Expression and the Role of TFF3 in Lung Adenocarcinoma Based on Bioinformatics Analysis

作　　者：王志远 孟玲 石娟 刘圆圆 李瑞頔 李文鑫 安淑红 WANG Zhiyuan;MENG Ling;SHI Juan(Department of Human Anatomy,School of Clinical and Basic Medicine(Institute of Basic Medicine),Shandong First Medical University(Shandong Academy of Medical Sciences),Shandong 250000,China)

机构地区：[1]山东第一医科大学(山东省医学科学院)临床与基础医学院人体解剖学教研室,济南250000 [2]山东第一医科大学第二附属医院呼吸内科,泰安271000 [3]阳信县职业中等学校医学技术系,滨州251800

出　　处：《医学研究杂志》2024年第10期110-117,122,共9页Journal of Medical Research

基　　金：山东省医药卫生科技发展计划项目(202003020569)。

摘　　要：目的采用生物信息学方法和体外细胞实验探索三叶因子3(trefoilfactor 3,TFF3)在肺腺癌中的作用。方法基于TIMER、UALCAN数据库分析TFF3在癌组织中的表达。基于TCGA数据库和R包,从临床特征、免疫微环境、肿瘤突变负荷、免疫治疗等多个角度分析TFF3高低表达患者组的特征差异。通过RTCA检测TFF3对NCI-H1975细胞数目的影响,通过Western blot法检测PI_(3)K/Akt信号通路Akt及其激活状态p-Akt的表达。结果TFF3在肺腺癌组织中表达增高,表达量与肺腺癌的病理类型有关,但与患者预后无关。TFF3低表达与低肿瘤纯度相关。TFF3和肿瘤突变负荷、数种免疫检查点基因表达呈负相关。TFF3处理NCI-H1975细胞后,细胞总数上升。Western blot法检测结果表明,TFF3能激活p-Akt基因表达。结论TFF3在某些肺腺癌亚型中表达升高,且与肺腺癌肿瘤免疫具有一定关联。TFF3可促进肺腺癌细胞增殖,并可能通过激活PI_(3)K/Akt信号通路发挥作用。TFF3可能成为肺腺癌患者一个新的治疗靶点。Objective To explore the role of TFF3 in lung adenocarcinoma using bioinformatics methods and in vitro cell experiments.Methods We analyzed the characteristic differences between patient groups with high and low TFF3 expression using the TCGA database and R package.The analysis covered various perspectives,including clinical features,immune microenvironment,tumor mutation burden,and immunotherapy.The effect of TFF3 on the number of NCI-H1975 cells was determined using RTCA,while the expression of the PI_(3)K/Akt signaling pathway Akt and its activated state p-Akt was detected using Western blot.Results The expression of TFF3 increased in lung adenocarcinoma tissue,and this increase was related to the pathological type of lung adenocarcinoma.However,it was not associated with the prognosis of patients.Low expression of TFF3 was associated with low tumor purity.Additionally,TFF3 showed a negative correlation with tumor mutation burden and the expression of several immune checkpoint genes.In our study,we treated NCI-H1975 cells with TFF3 and observed an increase in the total number of cells.Furthermore,the results from Western blot analysis indicated that TFF3 could activate the expression of the p-Akt gene.Conclusion TFF3 could be upregulated in specific subtypes of lung adenocarcinoma and linked with tumor immunity of lung adenocarcinoma.We observed that TFF3 can enhance the growth of lung adenocarcinoma cells,possibly by activating the PI_(3)K/Akt signaling pathway.Our findings suggest that TFF3 could serve as a promising therapeutic target for individuals diagnosed with lung adenocarcinoma.

关 键 词：TFF3 肺腺癌 生物信息学分析 肿瘤免疫 PI_(3)K/Akt 

分 类 号：R734[医药卫生—肿瘤]