血浆cfDNA中HOXA6、FHIT甲基化水平在前列腺癌诊断中的作用  

The Role of HOXA6 and FHIT Methylation Levels in Plasma cfDNA in the Diagnosis of Prostate Cancer

作　　者：李丹 陈学栋 谭琳[1] LI Dan;CHEN Xuedong;TAN Lin(Urology Department Lishui People′s Hospital,Zhejiang 323050,China)

机构地区：[1]丽水市人民医院泌尿外科,323050

出　　处：《医学研究杂志》2024年第10期157-160,177,共5页Journal of Medical Research

基　　金：浙江省丽水市科技局计划项目(2020GYX22)。

摘　　要：目的 探索血浆中循环游离DNA(circulating free DNA,cfDNA)中HOXA6、脆性组氨酸三联体基因(fragile histidine triad gene, FHIT)甲基化水平及其在前列腺癌(prostate cancer, PCa)诊断中的作用。方法 选取2021年2月~2023年2月在笔者医院就诊的192例前列腺疾病患者作为研究对象,其中PCa患者112例作为疾病组,前列腺增生患者80例作为对照组。采用甲基化特异性PCR(methylation specific PCR,MSP)法对血浆cfDNA中HOXA6及FHIT的甲基化水平进行检测;分析血浆cfDNA中HOXA6及FHIT的甲基化水平与临床资料的关系;多因素Logistic分析PCa发生的影响因素;评价HOXA6及FHIT甲基化对PCa的诊断价值。结果 疾病组血浆cfDNA中HOXA6及FHIT甲基化率显著高于对照组(P<0.05);TNM分期在Ⅰ~Ⅱ期的患者HOXA6及FHIT甲基化率均低于Ⅲ~Ⅳ期患者,有淋巴结转移的患者HOXA6及FHIT甲基化率高于无淋巴结转移的患者(P<0.05);HOXA6、FHIT甲基化联合诊断PCa的敏感度为75.89%、特异性为95.00%,二者联合的诊断效能高于HOXA6、FHIT甲基化单独诊断的效能;多因素Logistic分析显示,HOXA6及FHIT甲基化是PCa发生的影响因素(P<0.05)。结论 PCa患者血浆cfDNA中HOXA6及FHIT甲基化水平升高,二者是PCa的影响因素,二者联合的诊断效能高于单独诊断的效能,具有一定的诊断价值。Objective To explore the methylation levels of HOXA6 and fragile histidine triad gene(FHIT)in circulating free DNA(cfDNA)in plasma and their role in the diagnosis of prostate cancer(PCa).Methods A total of 192 prostate illness patients who visited our hospital from February 2021 to February 2023 were collected as the study subjects,including 112 PCa patients as the disease group and 80 patients with prostatic hyperplasia as the control group.The methylation specific PCR(MSP)method was applied to detect the methylation levels of HOXA6 and FHIT in plasma cfDNA;the relationship between the methylation levels of HOXA6 and FHIT in plasma cfDNA and clinical data was analyzed;multi-factor Logistic was applied to analyze the influencing factors of PCa occurrence;and the diagnostic value of HOXA6 and FHIT methylation in PCa was evaluated.Results The methylation rates of HOXA6 and FHIT in the plasma cfDNA of the disease group were significantly higher than those of the control group(P<0.05);the methylation rates of HOXA6 and FHIT in patients with TNM stagesⅠ-Ⅱwere lower than those in stagesⅢ-Ⅳ,while patients with lymph node metastasis had higher HOXA6 and FHIT methylation rates than those without lymph node metastasis(P<0.05);the sensitivity and specificity of the combination of HOXA6 and FHIT methylation in the diagnosis of PCa were 75.89%and 95.00%,respectively,the diagnostic efficacy of the combination of the two was higher than that of HOXA6 and FHIT methylation alone;multivariate Logistic analysis showed that HOXA6 and FHIT methylation were the influencing factors for the occurrence of PCa(P<0.05).Conclusion The levels of HOXA6 and FHIT methylation in plasma cfDNA of PCa patients are elevated,they could be the influencing factors of PCa,and the combination of the two factors has higher diagnostic efficacy than individual diagnosis,and has certain diagnostic value.

关 键 词：前列腺癌 甲基化 cfDNA HOXA6 FHIT 

分 类 号：R737.1[医药卫生—肿瘤]