益智清心方对记忆功能障碍小鼠的保护作用研究  

Protective Effect of Yizhi Qingxin Formula on Mice with Memory Dysfunction

作　　者：姚久秀 魏微 曹宇[3] 罗奕苗 李浩 YAO Jiuxiu;WEI Wei;CAO Yu;LUO Yimiao;LI Hao(Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijing 100020,China;Shandong University of Traditional Chinese Medicine,Ji′nan 250014,China;Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)

机构地区：[1]中国中医科学院望京医院,北京100020 [2]山东中医药大学,济南250014 [3]中国中医科学院西苑医院,北京100091

出　　处：《世界中医药》2024年第18期2743-2748,2754,共7页World Chinese Medicine

基　　金：国家重点研发计划“中医药现代化”重点专项(2022YFC3501400)——基于“虚损瘀毒”病机基础的阿尔茨海默病中医诊疗规律系统化研究;国家自然科学基金项目(82104675)——参麻益智方干预小胶质细胞与血脑屏障互作治疗血管性痴呆的机制研究;中国中医科学院科技创新工程项目(CI2021A04618)——益智清心方干预衰老小胶质细胞治疗AD的作用机制研究;中国中医科学院科技创新工程项目(CI2021A01401)——老年期痴呆的创新中药与关键技术研究;“优势病种-医院制剂-新药”研发专项(ZZ15-XY-PT-02)——治疗阿尔茨海默病的中药复方新药—益智清心颗粒的研究与开发。

摘　　要：目的:评价益智清心方对小鼠记忆功能障碍的影响。方法:在益智清心方给药15 d后,分别于训练前、训练后以及测试前选取96只ICR小鼠分别采用东莨菪碱、亚硝酸钠和乙醇建立记忆获得障碍、记忆巩固障碍和记忆再现障碍小鼠模型,各32只。按随机数字表法分对照组、模型组、益智清心方低剂量组和益智清心方高剂量组,每组8只。采用旷场实验、新物体识别实验和跳台实验评估各组小鼠运动能力,以及益智清心方对记忆获得障碍、记忆巩固障碍和记忆再现障碍的影响。此外,运用苏木精-伊红(HE)染色观察益智清心方对学习记忆障碍小鼠脑组织病理形态影响。结果:旷场实验未发现各组小鼠运动能力的差异。新物体识别实验表明,益智清心方低、高剂量组小鼠较模型组对新物体的偏好指数和识别系数均明显升高。跳台实验显示,益智清心方低、高剂量组较模型组小鼠的错误次数减少,逃避潜伏期延长。海马区HE染色显示.与模型组比较,益智清心方低、高剂量组锥体细胞层细胞带结构较完整,分层明显,细胞排列紧密,神经元体积较大,形态较规则,结构清晰,胞浆透明。结论:益智清心方可以改善记忆损伤模型动物的学习记忆能力。Objective:To evaluate the effects of Yizhi Qingxin Formula on memory dysfunction in mice.Methods:After 15 days of administration of Yizhi Qingxin Formula,96 ICR mice were selected before training,after training,and before testing to establish mouse models of memory acquisition impairment,memory consolidation impairment,and memory retrieval impairment using scopolamine,sodium nitrite,and ethanol,respectively,with 32 mice in each model.Mice were randomly divided into control group,model group,low-dose Yizhi Qingxin Formula group,and high-dose Yizhi Qingxin Formula group,with 8 mice in each group.The open field test,novel object recognition test,and step-down test were used to assess the motor ability of the mice,as well as the effects of Yizhi Qingxin Formula on memory acquisition,consolidation,and retrieval impairments.Additionally,hematoxylin and eosin(HE)staining was used to observe the effects of Yizhi Qingxin Formula on the brain histopathology of mice with learning and memory impairment.Results:The open field test showed no significant difference in motor ability among the groups.The novel object recognition test indicated that the preference index and recognition coefficient for the new object were significantly higher in the low-dose and high-dose Yizhi Qingxin Formula groups than in the model group.The step-down test showed that the number of errors decreased,and the latency to avoid the platform increased in both the low-dose and high-dose Yizhi Qingxin Formula groups compared to the model group.HE staining of the hippocampus revealed that,compared to the results in the model group,the pyramidal cell layers in the low-dose and high-dose Yizhi Qingxin Formula groups were more intact,with clear stratification,tightly arranged cells,larger neurons,regular morphology,clear structure,and transparent cytoplasm.Conclusion:Yizhi Qingxin Formula can improve the learning and memory abilities of animals with memory dysfunction.

关 键 词：益智清心方 记忆障碍 记忆获得 记忆巩固 记忆再现 东莨菪碱 亚硝酸钠 乙醇 

分 类 号：R25[医药卫生—中医内科学]