赛北紫堇正丁醇部位化学成分及其抗氧化活性研究  

Chemical constituents from n-butanol fraction of Corydalis impatiens and their antioxidant activities

作　　者：邓超凡 南泽东 王真真 杨静玲 安振宇 吴秀丽[2] 马晓莉 江志波 DENG Chao-fan;NAN Ze-dong;WANG Zhen-zhen;YANG Jing-ling;AN Zhen-yu;WU Xiu-li;MA Xiao-li;JIANG Zhi-bo(School of Chemistry and Chemical Engineering,Key Laboratory of Chemical Engineering and Technology of State Ethnic Affairs Commission,North Minzu University,Yinchuan 750021,China;College of Pharmacy,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]北方民族大学化学与化学工程学院,国家民委化工技术重点实验室,宁夏银川750021 [2]宁夏医科大学药学院,宁夏银川750004

出　　处：《中成药》2024年第11期3671-3676,共6页Chinese Traditional Patent Medicine

基　　金：宁夏自然科学基金一般项目(2022AAC03292);宁夏重点研发项目(2021BEB04019);宁夏自然科学基金项目优秀青年项目(2022AAC05041,2023AAC05048);北方民族大学科研启动项目(2021KYQD35)。

摘　　要：目的研究赛北紫堇正丁醇部位的化学成分及其抗氧化活性。方法正丁醇部位采用硅胶、ODS、MCI、Sephadex LH-20及半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用DPPH法和酪氨酸酶法测定抗氧化活性。结果从中分离得到14个化合物,分别鉴定为烟酰胺(1)、L-焦谷氨酸甲酯(2)、bungeanoline F(3)、富马酸单甲酯(4)、5-羟甲基糠醛(5)、对羟基苯甲酸(6)、hydroxybenzoate(7)、原儿茶酸甲酯(8)、阿魏酸甲酯(9)、二甲基咖啡酸(10)、dimethyl feruloyl malate(11)、(-)-4-O-阿魏酰奎宁酸甲酯(12)、丁香酯素(13)和黑麦草内酯(14)。化合物1、8、11和13的DPPH自由基清除能力较强,IC 50值为54.47~97.4μmol/L;化合物13对酪氨酸酶有潜在抑制作用。结论化合物4~14为首次从紫堇属中分离得到,化合物3为首次从该植物中分离得到。化合物1、8、11和13具有较强的抗氧化活性。AIM To study the chemical constituents from n-butanol fraction of Corydalis impatiens(Pall.)Fisch.and their antioxidant activities.METHODS The n-butanol fraction was isolated and purified by silica gel,MCI,ODS,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antioxidant activities were determined by DPPH method and tyrosinase method.RESULTS Fourteen compounds were isolated and identified as nicotinamide(1),methyl L-pyroglutamate(2),bungeanoline F(3),monomethyl fumarate(4),5-hydroxymethylfurfural(5),4-hydroxybenzoic acid(6),hydroxybenzoate(7),methyl 3,4-dihydroxybenzoate(8),methyl ferulate(9),dimethylcaffeic acid(10),dimethyl feruloyl malate(11),(-)-4-O-feruloylquinic acid(12),syringaresinol(13)and(-)-loliolide(14).Compounds 1,8,11 and 13 showed strong antioxidant activites on DPPH free radicals,with IC 50 values ranging from 54.47 to 97.4μmol/L.Compound 13 had potential inhibitory effect on tyrosinase.CONCLUSION Compounds 4-14 are first isolated from Corydalis genus,and 3 is isolated from this plant for the first time.Compounds 1,8,11 and 13 have strong antioxidant activities.

关 键 词：赛北紫堇 正丁醇部位 化学成分 分离鉴定 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学]