Antibacterial activity evaluation of a novel K3-specific phage against Acinetobacter baumannii and evidence for receptor-binding domain transfer across morphologies  

作　　者：Xiangkuan Zheng Meihan Liu Pei Li Sixiang Xu Long Chen Guoxin Xu Xiaoxiao Pang Hong Du Yishan zheng Xiang Huo Zhongming Tan Juan Li Zhirong Li Wei Zhang 

机构地区：[1]College of Veterinary Medicine,Nanjing Agricultural University,Key Lab of Animal Bacteriology,Ministry of Agriculture and Rural Affairs,Nanjing,210095,China [2]Department of Clinical Laboratory,Zhangjiagang Hospital Affiliated to Soochow University,Zhangjiagang,215600,China [3]Department of Clinical Laboratory,The Second Affiliated Hospital of Soochow University,Suzhou,215004,China [4]Intensive Care Unit,The Second Hospital of Nanjing,Nanjing University of Chinese Medicine,Nanjing,210003,China [5]Jiangsu Provincial Center for Disease Prevention and Control,Nanjing,210009,China [6]National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Disease,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,102206,China [7]Provincial Center for Clinical Laboratories,The Second Hospital of Hebei Medical University,Shijiazhuang,050000,China

出　　处：《Virologica Sinica》2024年第5期767-781,共15页中国病毒学（英文版）

基　　金：supported by a grant from the NHC Key laboratory of Enteric Pathogenic Microbiology(Jiangsu Provincial Center for Disease Control and Prevention,EM202303);Guizhou Province Science and Technology Plan Project(Grant No.QKH[2023]008);the Science Foundation of Jiangsu Province Health Department(ZDB2020014);National Natural Science Foundation of China(82002108);Science and Technology Program of Suzhou(SKYD2023050);Suzhou Municipal Health Commission(KJXW2023061).

摘　　要：Acinetobacter baumannii(A.baumannii)poses a serious public health challenge due to its notorious antimicrobial resistance,particularly carbapenem-resistant A.baumannii(CRAB).In this study,we isolated a virulent phage,named P1068,from medical wastewater capable of lysing CRAB,primarily targeting the K3 capsule type.Basic characterization showed that P1068 infected the A.baumannii ZWAb014 with an optimal MOI of 1,experienced a latent period of 10 min and maintained stability over a temperature range of 4–37C and pH range of 3–10.Phylogenetic and average nucleotide identity analyses indicate that P1068 can be classified as a novel species within the genus Obolenskvirus of the Caudoviricetes class as per the most recent virus classification released by the International Committee on Taxonomy of Viruses(ICTV).Additionally,according to classical morphological classification,P1068 is identified as a T4-like phage(Myoviridae).Interestingly,we found that the tail fiber protein(TFP)of P1068 shares 74%coverage and 88.99%identity with the TFP of a T7-like phage(Podoviridae),AbKT21phiIII(NC_048142.1).This finding suggests that the TFP gene of phages may undergo horizontal transfer across different genera and morphologies.In vitro antimicrobial assays showed that P1068 exhibited antimicrobial activity against A.baumannii in both biofilm and planktonic states.In mouse models of intraperitoneal infection,P1068 phage protected mice from A.baumannii infection and significantly reduced bacterial loads in various tissues such as the brain,blood,lung,spleen,and liver compared to controls.In conclusion,this study demonstrates that phage P1068 might be a potential candidate for the treatment of carbapenem-resistant and biofilmforming A.baumannii infections,and expands the understanding of horizontal transfer of phage TFP genes.

关 键 词：PHAGE A.baumannii CARBAPENEM-RESISTANT Capsule type Tail fiber protein Antibacterial activity 

分 类 号：R373[医药卫生—病原生物学]