Single-cell RNA-sequencing reveals a profound immune cell response in human cytomegalovirus-infected humanized mice  

作　　者：An Wang Xiao-Xu Zhu Yuanyuan Bie Bowen Zhang Wenting Ji Jing Lou Muhan Huang Xi Zhou Yujie Ren 

机构地区：[1]Key Laboratory of Virology and Biosafety,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan,430071,China [2]State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan,430071,China [3]University of Chinese Academy of Sciences,Beijing,100049,China [4]School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,230026,China

出　　处：《Virologica Sinica》2024年第5期782-792,共11页中国病毒学（英文版）

基　　金：supported by the National Key R&D Program of China(2023YFC23066000 to Y.R.);the National Natural Science Foundation of China(32100106 to Y.R.,and U21A20423 and 32225004 to X.Z.);the CAS Youth Innovation Promotion Association(2023351 to Y.R.);Hubei Province Natural Science Funds(2023AFB582 to Y.R.and 2023AFA008 to X.Z);the Fund of the Science and Technology Bureau of Wuhan(2023020201010086 to Y.R.).

摘　　要：Human cytomegalovirus(HCMV)is a common herpesvirus that persistently infects a large portion of the world's population.Despite the robust host immune response,HCMV is able to replicate,evade host defenses,and establish latency throughout the lifespan by developing multiple immunomodulatory strategies,making the studies on the interaction between HCMV infection and host response particularly important.HCMV has a strict host specificity that specifically infects humans.Therefore,most of the in vivo researches of HCMV rely on clinical samples.Fortunately,the establishment of humanized mouse models allows for convenient in-lab animal experiments involving HCMV infection.Single-cell RNA sequencing enables the study of the relationship between viral and host gene expressions at the single-cell level within host cells.In this study,we assessed the gene expression alterations of PBMCs at the single-cell level within HCMV-infected humanized mice,which sheds light onto the virus-host interactions in the context of HCMV infection of humanized mice and provides a valuable dataset for the related researches.

关 键 词：Human cytomegalovirus(HCMV) Single-cell RNA-Sequencing PBMC Viral infection 

分 类 号：R-332[医药卫生]